Bcl-11b Antibody (F-4): sc-365320

Bcl-11b Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bcl-11b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Bcl-11b antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bcl-11b protein, also known as CTIP2 or RIT1, plays a pivotal role in lymphopoiesis and is crucial for T cell development and function. Bcl-11b′s ability to act as a transcriptional repressor maintains the balance of gene expression during lymphocyte development, and disruptions in Bcl-11b locus have been linked to T cell acute lymphoblastic leukemia. Bcl-11b interacts with metastasis-associated proteins MTA1 and MTA2, highlighting Bcl-11b′s involvement in complex regulatory networks that influence cell fate decisions. Bcl-11b′s significance extends beyond normal hematopoiesis, as Bcl-11b dysregulation can contribute to hematopoietic malignancies, making Bcl-11b a critical target for research and therapeutic interventions.

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Bcl-11b Antibody (F-4) References:

1. The configuration of the immunoglobulin genes in B cell chronic lymphocytic leukemia.  |  Dyer, MJ. and Oscier, DG. 2002. Leukemia. 16: 973-84. PMID: 12040429
2. COUP-TF (chicken ovalbumin upstream promoter transcription factor)-interacting protein 1 (CTIP1) is a sequence-specific DNA binding protein.  |  Avram, D., et al. 2002. Biochem J. 368: 555-63. PMID: 12196208
3. Bcl11a is essential for normal lymphoid development.  |  Liu, P., et al. 2003. Nat Immunol. 4: 525-32. PMID: 12717432
4. Bcl11: sibling rivalry in lymphoid development.  |  Durum, SK. 2003. Nat Immunol. 4: 512-4. PMID: 12774073
5. The cardiac homeobox gene NKX2-5 is deregulated by juxtaposition with BCL11B in pediatric T-ALL cell lines via a novel t(5;14)(q35.1;q32.2).  |  Nagel, S., et al. 2003. Cancer Res. 63: 5329-34. PMID: 14500364
6. Two distinct methods analyzing chromatin structure using centrifugation and antibodies to modified histone H3: both provide similar chromatin states of the Rit1/Bcl11b gene.  |  Togashi, T., et al. 2004. Biochem Biophys Res Commun. 313: 489-95. PMID: 14697215
7. Involvement of V(D)J recombinase in the generation of intragenic deletions in the Rit1/Bcl11b tumor suppressor gene in gamma-ray-induced thymic lymphomas and in normal thymus of the mouse.  |  Sakata, J., et al. 2004. Carcinogenesis. 25: 1069-75. PMID: 14754877
8. BCL11A-dependent recruitment of SIRT1 to a promoter template in mammalian cells results in histone deacetylation and transcriptional repression.  |  Senawong, T., et al. 2005. Arch Biochem Biophys. 434: 316-25. PMID: 15639232
9. Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo.  |  Arlotta, P., et al. 2005. Neuron. 45: 207-21. PMID: 15664173
10. Disruption of the BCL11B gene through inv(14)(q11.2q32.31) results in the expression of BCL11B-TRDC fusion transcripts and is associated with the absence of wild-type BCL11B transcripts in T-ALL.  |  Przybylski, GK., et al. 2005. Leukemia. 19: 201-8. PMID: 15668700
11. p53 prevents maturation of T cell development to the immature CD4-CD8+ stage in Bcl11b-/- mice.  |  Okazuka, K., et al. 2005. Biochem Biophys Res Commun. 328: 545-9. PMID: 15694382
12. Contingent gene regulatory networks and B cell fate specification.  |  Singh, H., et al. 2005. Proc Natl Acad Sci U S A. 102: 4949-53. PMID: 15788530
13. BCL11B functionally associates with the NuRD complex in T lymphocytes to repress targeted promoter.  |  Cismasiu, VB., et al. 2005. Oncogene. 24: 6753-64. PMID: 16091750

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bcl-11b Antibody (F-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Bcl-11b Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bcl-11b Antibody (F-4) Specifications

Alternate Product Name: BCL11B

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 251-290

Epitope Target: Bcl-11b

Epitope Species: human

Product Reactivity/Detection: Bcl-11b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Bcl-11b Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin