BATF2 Double Nickase Plasmid (h): sc-403270-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BATF2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BATF2 Double Nickase Plasmid (h) and BATF2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BATF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BATF2 Antibody (1B11): sc-293274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BATF2 Double Nickase Plasmid (h) | sc-403270-NIC | 20 µg | $410.00 | |||
BATF2 Double Nickase Plasmid (h2) | sc-403270-NIC-2 | 20 µg | $410.00 |
BATF2 (basic leucine zipper ATF-like transcription factor 2) is an interferon-inducible member of the AP-1 transcription factor network that modulates inflammatory gene expression programs and innate immune signaling. In human cells, BATF2 participates in transcriptional regulation downstream of cytokine and pathogen-sensing pathways, influencing macrophage activation states, antigen presentation, and stress-responsive transcriptional outputs. Its activity has been linked to control of cell proliferation and differentiation through context-dependent interactions with other bZIP factors, shaping immune–tumor microenvironment crosstalk. Dysregulated BATF2 expression has been reported across multiple disease settings, including cancer and chronic inflammatory conditions, where it is used to interrogate interferon-driven transcriptional states and immune regulation mechanisms.
BATF2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BATF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BATF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BATF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BATF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.