Basic Cytokeratin Antibody (AE3): sc-57004

Basic Cytokeratin Antibody (AE3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Basic Cytokeratin protein of mouse, rat, human, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Basic Cytokeratin antibody (AE3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratins, a diverse group of intermediate filament proteins, are crucial for maintaining the structural integrity of epithelial cells, as they are expressed in both keratinized and non-keratinized epithelial tissues. Basic Cytokeratin proteins play a vital role in cellular differentiation and tissue specialization, which are essential for proper organ function and repair mechanisms. Basic Cytokeratin (AE3) monoclonal antibody serves as an important marker for identifying the origin of metastatic tumors, aiding in cancer diagnosis and treatment strategies. The genes encoding human type II/basic cytokeratins are clustered on chromosome 12q, and their expression is typically initiated in differentiating epithelia, highlighting their significance in the development and maintenance of epithelial tissues. The formation of intermediate filaments necessitates the pairing of at least one acidic and one basic cytokeratin, underscoring the intricate interplay between different cytokeratin types in cellular architecture.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Basic Cytokeratin Antibody (AE3) References:

1. Murine oviduct epithelial cell cytokine responses to Chlamydia muridarum infection include interleukin-12-p70 secretion.  |  Johnson, RM. 2004. Infect Immun. 72: 3951-60. PMID: 15213139
2. Cytokeratin expression in primary epithelial cell culture from bovine conjunctiva.  |  Paladino, G., et al. 2004. Tissue Cell. 36: 323-32. PMID: 15385149
3. Inherited defects in keratins.  |  Irvine, AD. 2005. Clin Dermatol. 23: 6-14. PMID: 15708284
4. Cytokeratins and dermatology.  |  Jacques, C., et al. 2005. Skinmed. 4: 354-60; quiz 360-1. PMID: 16276151
5. Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12.  |  Rosenberg, M., et al. 1991. Cytogenet Cell Genet. 57: 33-8. PMID: 1713141
6. Best practices in diagnostic immunohistochemistry: pleomorphic cutaneous spindle cell tumors.  |  Folpe, AL. and Cooper, K. 2007. Arch Pathol Lab Med. 131: 1517-24. PMID: 17922587
7. Co-expression of specific acid and basic cytokeratins in teratocarcinoma-derived fibroblasts treated with 5-azacytidine.  |  Darmon, M. 1985. Dev Biol. 110: 47-52. PMID: 2408944
8. A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells.  |  Schiller, DL., et al. 1982. EMBO J. 1: 761-9. PMID: 6203737
9. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
10. Keratins and the skin.  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
11. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
12. Molecular characterization of a tissue-polypeptide-specific-antigen epitope and its relationship to human cytokeratin 18.  |  Rydlander, L., et al. 1996. Eur J Biochem. 241: 309-14. PMID: 8917424

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Basic Cytokeratin Antibody (AE3) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Basic Cytokeratin Antibody (AE3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Basic Cytokeratin Antibody (AE3) Specifications

Alternate Product Name: Cytokeratins CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8

Product Clone Name: AE3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Basic Cytokeratin

Epitope Species: human

Product Reactivity/Detection: basic (type II) cytokeratins

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Basic Cytokeratin Antibody (AE3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin