BAI-2 CRISPR/Cas9 KO Plasmid (h): sc-406051
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAI-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BAI-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BAI-2 Antibody (6A12): sc-517130
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAI-2 CRISPR/Cas9 KO Plasmid (h) | sc-406051 | 20 µg | $397.00 |
Overview
ADGRB2 encodes the adhesion G protein–coupled receptor BAI-2 (brain-specific angiogenesis inhibitor 2), a multi-domain membrane receptor enriched in the nervous system and implicated in cell–cell and cell–matrix interactions. As an adhesion GPCR, BAI-2 integrates extracellular cues with intracellular signaling programs that shape cytoskeletal organization, membrane dynamics, and cellular communication, contributing to processes such as neuronal development and tissue homeostasis. Dysregulated adhesion GPCR signaling has been linked to altered angiogenic and inflammatory microenvironments and to changes in cellular motility and differentiation states. Accordingly, ADGRB2/BAI-2 is studied in contexts including neurobiology and tumor-associated signaling networks where adhesion-dependent pathways influence disease-relevant phenotypes.
BAI-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ADGRB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADGRB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADGRB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BAI-2 protein expression.
This CRISPR knockout system enables efficient generation of ADGRB2-deficient cell models for investigation of BAI-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ADGRB2 exon(s) critical for BAI-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ADGRB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BAI-2 CRISPR/Cas9 KO Plasmid (h) and BAI-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADGRB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BAI-2 HDR Plasmid (h) and BAI-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADGRB2 homology arms to support homology-directed repair at defined ADGRB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.