Bag-3 CRISPR/Cas9 KO Plasmid (h): sc-402929
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Bag-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Bag-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Bag-3 Antibody (19): sc-136467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bag-3 CRISPR/Cas9 KO Plasmid (h) | sc-402929 | 20 µg | $397.00 |
Overview
BAG3 encodes the co-chaperone Bag-3, a stress-inducible regulator of protein quality control that partners with HSP70 and small heat shock proteins to coordinate chaperone-assisted selective autophagy and proteostasis. Bag-3 influences apoptosis and cell survival signaling by modulating BCL2 family interactions and linking cytoskeletal dynamics to stress responses through pathways including autophagy–lysosome function and ubiquitin-dependent turnover. In human cells, BAG3 activity is implicated in maintaining mitochondrial and sarcomeric integrity and in regulating responses to proteotoxic, mechanical, and oxidative stress. Dysregulation of BAG3-associated networks has been connected to cardiomyopathy, neurodegenerative disease mechanisms, and cancer-relevant stress adaptation, making it a useful node for studying homeostasis and remodeling under stress.
Bag-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BAG3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BAG3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BAG3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Bag-3 protein expression.
This CRISPR knockout system enables efficient generation of BAG3-deficient cell models for investigation of Bag-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BAG3 exon(s) critical for Bag-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BAG3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Bag-3 CRISPR/Cas9 KO Plasmid (h) and Bag-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BAG3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Bag-3 HDR Plasmid (h) and Bag-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BAG3 homology arms to support homology-directed repair at defined BAG3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.