Bag-1 Antibody (E-11): sc-376848

Bag-1 Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Bag-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Bag-1 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bag-1 plays a crucial role in regulating apoptosis, acting as a protective factor that enhances the anti-apoptotic effects of Bcl-2. By binding to Bcl-2, Bag-1 stabilizes its function, thereby preventing cell death under stress conditions. This interaction is particularly important in the context of cancer, where dysregulation of apoptosis can lead to tumor survival and resistance to therapy. Furthermore, Bag-1 has been implicated in various cellular processes, including cell proliferation and differentiation, highlighting its significance in both normal physiology and disease states. The ability of Bag-1 to modulate the apoptotic pathway suggests potential therapeutic targeting for enhancing cancer treatment efficacy, making Bag-1 (E-11) monoclonal antibody an essential tool for researchers studying apoptosis and related signaling pathways.

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Bag-1 Antibody (E-11) References:

1. Ubiquitylation of BAG-1 suggests a novel regulatory mechanism during the sorting of chaperone substrates to the proteasome.  |  Alberti, S., et al. 2002. J Biol Chem. 277: 45920-7. PMID: 12297498
2. Regulation of BAG-1 IRES-mediated translation following chemotoxic stress.  |  Dobbyn, HC., et al. 2008. Oncogene. 27: 1167-74. PMID: 17700523
3. BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-kappaB activity.  |  Clemo, NK., et al. 2008. Carcinogenesis. 29: 849-57. PMID: 18204076
4. Overexpressed nuclear BAG-1 in human hepatocellular carcinoma is associated with poor prognosis and resistance to doxorubicin.  |  Ni, W., et al. 2013. J Cell Biochem. 114: 2120-30. PMID: 23553841
5. Bag-1 promotes cell survival through c-Myc-mediated ODC upregulation that is not preferred under apoptotic stimuli in MCF-7 cells.  |  Ozfiliz, P., et al. 2015. Cell Biochem Funct. 33: 293-307. PMID: 26178413
6. Silencing Bag-1 gene via magnetic gold nanoparticle-delivered siRNA plasmid for colorectal cancer therapy in vivo and in vitro.  |  Huang, W., et al. 2016. Tumour Biol. 37: 10365-74. PMID: 26846101
7. BAG-1/SODD, HSP70, and HSP90 are potential prognostic markers of poor survival in node-negative breast carcinoma.  |  Davidson, B., et al. 2016. Hum Pathol. 54: 64-73. PMID: 27038683
8. An increase in BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signalling.  |  Lin, PL., et al. 2017. Eur J Cancer. 85: 95-105. PMID: 28892778
9. Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1.  |  Wang, HG., et al. 1996. Proc Natl Acad Sci U S A. 93: 7063-8. PMID: 8692945
10. Role of Bag-1 in the survival and proliferation of the cytokine-dependent lymphocyte lines, Ba/F3 and Nb2.  |  Clevenger, CV., et al. 1997. Mol Endocrinol. 11: 608-18. PMID: 9139804

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bag-1 Antibody (E-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Bag-1 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bag-1 Antibody (E-11) Specifications

Alternate Product Name: Bag-1

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 117-155

Epitope Target: Bag-1

Epitope Species: human

Product Reactivity/Detection: Bag-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Bag-1 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Bag-1 antibody is Bag-1 Antibody (3.10G3E2): sc-56003.