Bag-1 Antibody (3.10G3E2): sc-56003

Bag-1 Antibody (3.10G3E2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Bag-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Bag-1 (3.10G3E2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bag-1 protein plays a crucial role in regulating apoptosis, acting as a Bcl-2-binding protein that enhances the protective effects of Bcl-2 against cell death. This interaction suggests that Bag-1 may be essential in modulating apoptotic pathways, particularly in contexts where Bcl-2 alone is insufficient to prevent apoptosis. By stabilizing Bcl-2, Bag-1 not only contributes to cell survival but also indicates that the mechanisms of apoptosis may involve a more complex interplay between various proteins than previously understood. The ability of Bag-1 to influence cell fate underscores its potential as a target for therapeutic interventions in diseases characterized by dysregulated apoptosis, such as cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Bag-1 Antibody (3.10G3E2) References:

1. Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells.  |  Ishioka, S., et al. 2006. Int J Med Sci. 4: 36-44. PMID: 17299580
2. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
3. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
4. The Bag-1 inhibitor, Thio-2, reverses an atypical 3D morphology driven by Bag-1L overexpression in a MCF-10A model of ductal carcinoma in situ.  |  Papadakis, ES., et al. 2016. Oncogenesis. 5: e215. PMID: 27043661
5. Over-expression of BAG-1 in head and neck squamous cell carcinomas (HNSCC) is associated with cisplatin-resistance.  |  Liu, S., et al. 2017. J Transl Med. 15: 189. PMID: 28877725
6. Bag-1-mediated HSF1 phosphorylation regulates expression of heat shock proteins in breast cancer cells.  |  Kizilboga, T., et al. 2024. FEBS Open Bio. 14: 1559-1569. PMID: 39049197
7. Induction of apoptosis by the Bcl-2 homologue Bak.  |  Chittenden, T., et al. 1995. Nature. 374: 733-6. PMID: 7715730
8. Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.  |  Kiefer, MC., et al. 1995. Nature. 374: 736-9. PMID: 7715731
9. Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity.  |  Takayama, S., et al. 1995. Cell. 80: 279-84. PMID: 7834747
10. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death.  |  Yang, E., et al. 1995. Cell. 80: 285-91. PMID: 7834748
11. BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax.  |  Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
12. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bag-1 Antibody (3.10G3E2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Bag-1 Antibody (3.10G3E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bag-1 Antibody (3.10G3E2) Specifications

Alternate Product Name: Bag-1

Product Clone Name: 3.10G3E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Bag-1

Epitope Species: human

Product Reactivity/Detection: Bag-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Bag-1 Antibody (3.10G3E2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin