BACH1 CRISPR Activation Plasmid (m): sc-419283-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BACH1 CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- BACH1 CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by BACH1 CRISPR Activation Plasmid (m) and BACH1 CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Bach1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BACH1 Antibody (L-25): sc-100995
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BACH1 CRISPR Activation Plasmid (m) | sc-419283-ACT | 20 µg | $397.00 |
Bach1 encodes the BTB and CNC homology 1 transcription factor (BACH1), a heme-responsive regulator that modulates oxidative stress programs by partnering with small Maf proteins and binding Maf recognition elements in target promoters. In mouse cells, BACH1 functions as a key node in redox homeostasis, influencing expression of cytoprotective genes such as Hmox1 and coordinating responses to reactive oxygen species, iron/heme metabolism, and inflammatory signaling. Through these mechanisms, BACH1 intersects with pathways controlling mitochondrial function, cell fate decisions, and stress-induced transcriptional remodeling. Dysregulated BACH1 activity has been associated with altered oxidative damage responses and has been studied in contexts relevant to inflammation, neurodegeneration, and tumor biology as a regulator of cellular adaptation to stress.
BACH1 CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Bach1 expression without altering the underlying DNA sequence.
BACH1 CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Bach1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Bach1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous BACH1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Bach1 locus and enabling the study of BACH1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of BACH1 pathway restoration in tumor cells with silenced or reduced Bach1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.