BACE HDR Plasmid (h): sc-401500-HDR. Western blot analysis of BACE expression in non-transfected control(A) and puromycin treated, BACE CRISPR/Cas9 KO Plasmid: sc-401500 plus BACE HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with BACE (A-12): sc-365948. β-Actin (C4): sc-47778 used as specificity and loading control.
BACE HDR Plasmid (h): sc-401500-HDR. Western blot analysis of BACE expression in non-transfected control(A) and puromycin treated, BACE CRISPR/Cas9 KO Plasmid: sc-401500 plus BACE HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with BACE (A-12): sc-365948. β-Actin (C4): sc-47778 used as specificity and loading control.
CRISPR/Cas9 KO Plasmids consists of BACE-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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CRISPR/Cas9 KO Plasmid
Recommended Control Products
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BACE Antibodies for analysis of cellular responses to BACE CRISPR Products
CRISPR/Cas9 KO plasmids, Double Nickase Plasmids and CRISPR/dCas9 Activation plasmids are considered a "Licensed Product" and are to be used in accordance with the Limited/Label License. Click here for details