B7RP-1 CRISPR/Cas9 KO Plasmid (h): sc-406855
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- B7RP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the B7RP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B7RP-1 CRISPR/Cas9 KO Plasmid (h) | sc-406855 | 20 µg | $397.00 |
Overview
ICOSLG encodes the inducible T-cell costimulator ligand (B7RP-1), a B7 family immune checkpoint molecule expressed by antigen-presenting cells and stromal compartments that engages ICOS on activated T cells. This interaction promotes costimulatory signaling that supports T-cell proliferation, survival, and cytokine programs, shaping germinal center reactions, T follicular helper differentiation, and class-switched antibody responses. ICOSLG–ICOS signaling intersects with PI3K-AKT–dependent pathways and modulates crosstalk between innate and adaptive immune cells within inflamed tissues and tumor microenvironments. Dysregulated ICOSLG expression or signaling has been implicated in autoimmune inflammation, allergic disease, transplant immunobiology, and immune evasion mechanisms relevant to cancer and chronic infection research.
B7RP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ICOSLG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ICOSLG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ICOSLG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish B7RP-1 protein expression.
This CRISPR knockout system enables efficient generation of ICOSLG-deficient cell models for investigation of B7RP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ICOSLG exon(s) critical for B7RP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ICOSLG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by B7RP-1 CRISPR/Cas9 KO Plasmid (h) and B7RP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ICOSLG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by B7RP-1 HDR Plasmid (h) and B7RP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ICOSLG homology arms to support homology-directed repair at defined ICOSLG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.