B7-H3 Antibody (F-11): sc-376769
- B7-H3 Antibody (F-11) is a mouse monoclonal IgG2a κ B7-H3 antibody, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 166-465 mapping within an N-terminal extracellular domain of B7-H3 of human origin
- B7-H3 Antibody (F-11) is recommended for detection of B7-H3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-B7-H3 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for B7-H3 Antibody (F-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with B7-H3 Antibody (F-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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B7-H3 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects B7-H3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). B7-H3 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. B7-H3, also known as B7-homolog 3 or B7RP-2, is a member of the B7 immunoglobulin superfamily and plays a crucial role in regulating T cell activation and immune responses. B7-H3 is widely expressed in non-lymphoid tissues and interacts with a putative receptor on activated T cells, distinct from other well-known costimulatory molecules such as CD28, CTLA-4, ICOS, and PD-1. B7-H3 costimulates proliferation of both CD4+ and CD8+ T cells, which is vital for effective immune responses. B7-H3′s ability to enhance Th1 and cytotoxic T cell responses suggests potential antitumor activity, making B7-H3 a promising target for therapeutic interventions in cancer treatment. B7-H3′s interactions may play a significant role in modulating cell-mediated immune responses against tumors, highlighting B7-H3′s importance in developing novel immunotherapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
B7-H3 Antibody (F-11) References:
- B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. | Chapoval, AI., et al. 2001. Nat Immunol. 2: 269-74. PMID: 11224528
- Characterization of mouse and human B7-H3 genes. | Sun, M., et al. 2002. J Immunol. 168: 6294-7. PMID: 12055244
- T lymphocytes express B7 family molecules following interaction with dendritic cells and acquire bystander costimulatory properties. | Ferlazzo, G., et al. 2002. Eur J Immunol. 32: 3092-101. PMID: 12385029
- The B7 family member B7-H3 preferentially down-regulates T helper type 1-mediated immune responses. | Suh, WK., et al. 2003. Nat Immunol. 4: 899-906. PMID: 12925852
- Mouse B7-H3 induces antitumor immunity. | Sun, X., et al. 2003. Gene Ther. 10: 1728-34. PMID: 12939639
- Murine B7-H3 is a negative regulator of T cells. | Prasad, DV., et al. 2004. J Immunol. 173: 2500-6. PMID: 15294965
- The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. | Suh, WK., et al. 2004. Proc Natl Acad Sci U S A. 101: 12969-73. PMID: 15317945
- B7-H3 promotes acute and chronic allograft rejection. | Wang, L., et al. 2005. Eur J Immunol. 35: 428-38. PMID: 15682454
- B7-H3: another molecule marker for Mo-DCs? | Zhang, G., et al. 2005. Cell Mol Immunol. 2: 307-11. PMID: 16274630
- mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion. | Liu, HJ., et al. 2023. Nat Commun. 14: 1214. PMID: 36869048
- Immune checkpoint B7-H3 is a therapeutic vulnerability in prostate cancer harboring PTEN and TP53 deficiencies. | Shi, W., et al. 2023. Sci Transl Med. 15: eadf6724. PMID: 37163614
- B7-H3 Inhibitors in Oncology Clinical Trials: A Review. | Feustel, K., et al. 2024. J Immunother Precis Oncol. 7: 53-66. PMID: 38327753
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B7-H3 Antibody (F-11) | sc-376769 | 200 µg/ml | $322.00 | |||
B7-H3 Antibody (F-11): m-IgGκ BP-HRP Bundle | sc-552438 | 200 µg Ab; 40 µg BP | $361.00 | |||
B7-H3 Antibody (F-11) AC | sc-376769 AC | 500 µg/ml, 25% agarose | $424.00 | |||
B7-H3 Antibody (F-11) HRP | sc-376769 HRP | 200 µg/ml | $322.00 | |||
B7-H3 Antibody (F-11) FITC | sc-376769 FITC | 200 µg/ml | $336.00 | |||
B7-H3 Antibody (F-11) PE | sc-376769 PE | 200 µg/ml | $349.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 488 | sc-376769 AF488 | 200 µg/ml | $364.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 546 | sc-376769 AF546 | 200 µg/ml | $364.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 594 | sc-376769 AF594 | 200 µg/ml | $364.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 647 | sc-376769 AF647 | 200 µg/ml | $364.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 680 | sc-376769 AF680 | 200 µg/ml | $364.00 | |||
B7-H3 Antibody (F-11) Alexa Fluor® 790 | sc-376769 AF790 | 200 µg/ml | $364.00 |