B7-1 Double Nickase Plasmid (m): sc-419570-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- B7-1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- B7-1 Double Nickase Plasmid (m) and B7-1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cd80. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: B7-1 Antibody (F-7): sc-376012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B7-1 Double Nickase Plasmid (m) | sc-419570-NIC | 20 µg | $410.00 |
Mouse Cd80 encodes the costimulatory ligand B7-1, a surface glycoprotein expressed primarily by antigen-presenting cells that modulates T cell priming and effector function through interactions with CD28 and CTLA-4. CD80 signaling contributes to immune synapse formation, cytokine production, and peripheral tolerance, integrating with pathways that shape adaptive immunity and inflammatory responses. Altered Cd80 expression is frequently studied in contexts of autoimmunity, chronic inflammation, transplantation immunity, and tumor immune evasion, where costimulatory balance influences disease phenotypes. As B7 family regulation intersects with antigen presentation and checkpoint biology, Cd80 is a common target for mechanistic studies of immune activation and suppression in mouse models.
B7-1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cd80 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cd80. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cd80 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cd80-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.