B7-1 Antibody (F-7): sc-376012

B7-1 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects B7-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-B7-1 antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. B7-1, also known as CD80, plays a crucial role in the immune response by providing essential costimulatory signals necessary for T cell activation. B7-1 is primarily located on the surface of antigen-presenting cells, where B7-1 interacts with CD28 and CTLA-4 receptors on T cells, thereby influencing T cell proliferation and cytokine production. This interaction is vital for the regulation of immune responses, as B7-1 helps ensure T cells are activated only in the presence of appropriate antigenic signals, preventing inappropriate immune activation that could lead to autoimmunity. B7-1′s ability to modulate T cell responses highlights B7-1′s importance in both immune regulation and potential therapeutic applications, such as in cancer immunotherapy, where enhancing or inhibiting these pathways can significantly impact treatment outcomes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

B7-1 Antibody (F-7) References:

1. Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor.  |  Shin, T., et al. 2003. J Exp Med. 198: 31-8. PMID: 12847135
2. Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy.  |  Schwartz, RH. 1992. Cell. 71: 1065-8. PMID: 1335362
3. Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.  |  Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
4. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo.  |  Paterson, AM., et al. 2011. J Immunol. 187: 1097-105. PMID: 21697456
5. B7-1 expression regulates the hypoxia-driven cytoskeleton rearrangement in glomerular podocytes.  |  Chang, JM., et al. 2013. Am J Physiol Renal Physiol. 304: F127-36. PMID: 23019228
6. Abatacept in B7-1-positive proteinuric kidney disease.  |  Alachkar, N., et al. 2014. N Engl J Med. 370: 1263-4. PMID: 24670180
7. Potential roles of the B7 and CD28 receptor families in autoimmunity and immune evasion.  |  Harlan, DM., et al. 1995. Clin Immunol Immunopathol. 75: 99-111. PMID: 7535672
8. Identification of residues in the V domain of CD80 (B7-1) implicated in functional interactions with CD28 and CTLA4.  |  Fargeas, CA., et al. 1995. J Exp Med. 182: 667-75. PMID: 7544393
9. Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28.  |  Peach, RJ., et al. 1995. J Biol Chem. 270: 21181-7. PMID: 7545666
10. A novel receptor involved in T-cell activation.  |  Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
11. CTLA4 mediates antigen-specific apoptosis of human T cells.  |  Gribben, JG., et al. 1995. Proc Natl Acad Sci U S A. 92: 811-5. PMID: 7846057
12. Vitamin D differentially regulates B7.1 and B7.2 expression on human peripheral blood monocytes.  |  Clavreul, A., et al. 1998. Immunology. 95: 272-7. PMID: 9824486

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-B7-1 Antibody (F-7) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-B7-1 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

B7-1 Antibody (F-7) Specifications

Alternate Product Name: CD80

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 35-242

Epitope Target: B7-1

Epitope Species: human

Product Reactivity/Detection: B7-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for B7-1 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin