B230216G23Rik CRISPR/Cas9 KO Plasmid (m): sc-435631
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- B230216G23Rik CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the B230216G23Rik genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B230216G23Rik CRISPR/Cas9 KO Plasmid (m) | sc-435631 | 20 µg | $397.00 |
Overview
Spx (B230216G23Rik) is a mouse gene that remains incompletely characterized, with evidence suggesting roles in transcriptional control and cellular homeostasis programs that influence stress adaptation and metabolic state. Like other poorly annotated loci, its expression and putative protein interactions are often investigated in the context of nucleus–cytoplasm signaling, RNA processing, and proteostasis networks that can shape cell-cycle behavior and differentiation potential. Variation in such regulatory nodes is frequently explored for links to phenotypes relevant to neurodevelopment, immune regulation, and susceptibility to complex disease traits in model organisms. Functional dissection of Spx is therefore useful for mapping gene networks, identifying pathway dependencies, and interpreting genotype-to-phenotype relationships in mouse-derived systems.
B230216G23Rik CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Spx gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Spx together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Spx open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish B230216G23Rik protein expression.
This CRISPR knockout system enables efficient generation of Spx-deficient cell models for investigation of B230216G23Rik signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Spx exon(s) critical for B230216G23Rik function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Spx genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by B230216G23Rik CRISPR/Cas9 KO Plasmid (m) and B230216G23Rik CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Spx locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by B230216G23Rik HDR Plasmid (m) and B230216G23Rik HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Spx homology arms to support homology-directed repair at defined Spx target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.