Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
B23/Nucleophosmin CRISPR/Cas9 KO Plasmid (m): sc-421943
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- B23/Nucleophosmin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the B23/Nucleophosmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: B23/Nucleophosmin Antibody (E-3): sc-271737
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
B23/Nucleophosmin CRISPR/Cas9 KO Plasmid (m) | sc-421943 | 20 µg | $397.00 |
Overview
Mouse Npm1 encodes B23/Nucleophosmin, a multifunctional nucleolar phosphoprotein that shuttles between the nucleolus, nucleus, and cytoplasm to coordinate ribosome biogenesis, rRNA processing, and nucleolar architecture. Nucleophosmin participates in cell-cycle control and stress responses through interactions with key regulatory networks, including p53/ARF signaling, DNA damage responses, and chromatin-associated processes. Its roles in centrosome duplication, genomic stability, and nucleocytoplasmic transport link Npm1 function to proliferative control and maintenance of cellular homeostasis. Dysregulated nucleophosmin activity and localization have been widely studied in cancer biology and hematopoietic regulation, making Npm1 a common target for mechanistic studies of oncogenic stress and nucleolar dysfunction.
B23/Nucleophosmin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Npm1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Npm1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Npm1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish B23/Nucleophosmin protein expression.
This CRISPR knockout system enables efficient generation of Npm1-deficient cell models for investigation of B23/Nucleophosmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Npm1 exon(s) critical for B23/Nucleophosmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Npm1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by B23/Nucleophosmin CRISPR/Cas9 KO Plasmid (m) and B23/Nucleophosmin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Npm1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by B23/Nucleophosmin HDR Plasmid (m) and B23/Nucleophosmin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Npm1 homology arms to support homology-directed repair at defined Npm1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.