B23/Nucleophosmin Antibody (FC82291): sc-56622

B23 Antibody (FC82291) is a mouse monoclonal IgG1 kappa light chain antibody that detects B23 (also known as nucleophosmin) in mouse, rat, human, bovine, and canine samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). B23 (FC82291) antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. B23 plays a crucial role in ribosomal assembly and ribosomal RNA transport, essential for protein synthesis in cells. B23 localization within the nucleolus is particularly important for ribosome biogenesis, and disruption in this process can lead to impaired cellular function and contribute to diseases such as cancer. B23 is highly phosphorylated by Cdc2 kinase during mitosis, suggesting involvement in cell cycle regulation. B23 interaction with proteins like karyopherin α and karyopherin β underscores its significance in nuclear transport, highlighting its multifaceted role in cellular homeostasis and maintenance of normal cellular functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-B23/Nucleophosmin Antibody (FC82291) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-B23/Nucleophosmin Antibody (FC82291) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

B23/Nucleophosmin Antibody (FC82291) Specifications

Alternate Product Name: NPM1

Product Clone Name: FC82291

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: B23

Epitope Species: rat

Product Reactivity/Detection: both the phosphorylated and the unphosphorylated B23 molecule

Species Reactivity/Detection: mouse, rat, human, bovine, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for B23/Nucleophosmin Antibody (FC82291): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred B23/Nucleophosmin antibody is B23/Nucleophosmin Antibody (0412): sc-47725.