B23/Nucleophosmin Antibody (0412): sc-47725

B23 Antibody (0412) is a mouse monoclonal IgG1 kappa light chain antibody that detects B23 (also known as nucleophosmin) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). B23 (0412) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. B23 plays a crucial role in ribosomal assembly and ribosomal RNA transport, essential processes for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in ribosomal subunit maturation, and proper localization is vital for maintaining efficient ribosome biogenesis. B23 is highly phosphorylated by Cdc2 kinase during mitosis, suggesting phosphorylation state may regulate function and interactions during cell division. Transport of proteins across the nuclear envelope is a selective, multi-step process requiring recognition of import substrates and docking at the nuclear pore complex, with B23 being a key player in this intricate mechanism.

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B23/Nucleophosmin Antibody (0412) References:

1. Nucleolar protein B23 has molecular chaperone activities.  |  Szebeni, A. and Olson, MO. 1999. Protein Sci. 8: 905-12. PMID: 10211837
2. B23 regulates GADD45a nuclear translocation and contributes to GADD45a-induced cell cycle G2-M arrest.  |  Gao, H., et al. 2005. J Biol Chem. 280: 10988-96. PMID: 15644315
3. A B23-interacting sequence as a tool to visualize protein interactions in a cellular context.  |  Lechertier, T., et al. 2007. J Cell Sci. 120: 265-75. PMID: 17179202
4. B23/nucleophosmin is involved in regulation of adenovirus chromatin structure at late infection stages, but not in virus replication and transcription.  |  Samad, MA., et al. 2012. J Gen Virol. 93: 1328-1338. PMID: 22337638
5. The Mechanism of Alisol B23 Acetate Inhibiting Lung Cancer: Targeted Regulation of CD11b/CD18 to Influence Macrophage Polarization.  |  Chen, Y., et al. 2022. Drug Des Devel Ther. 16: 3677-3689. PMID: 36277599
6. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
7. Cell cycle phase-dependent changes of localization and oligomerization states of nucleophosmin / B23.  |  Chou, YH. and Yung, BY. 1995. Biochem Biophys Res Commun. 217: 313-25. PMID: 8526928
8. Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.  |  Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
9. The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
10. Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.  |  Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
11. Decreased accumulation and dephosphorylation of the mitosis-specific form of nucleophosmin/B23 in staurosporine-induced chromosome decondensation.  |  Lu, YY., et al. 1996. Biochem J. 317 (Pt 1): 321-7. PMID: 8694782
12. Expression of nucleophosmin/B23 in normal and neoplastic colorectal mucosa.  |  Nozawa, Y., et al. 1996. J Pathol. 178: 48-52. PMID: 8778315

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-B23/Nucleophosmin Antibody (0412) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-B23/Nucleophosmin Antibody (0412) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

B23/Nucleophosmin Antibody (0412) Specifications

Alternate Product Name: NPM1

Product Clone Name: 0412

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: B23

Epitope Species: human

Product Reactivity/Detection: B23

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for B23/Nucleophosmin Antibody (0412): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin