B-Myb (h): 293T Lysate: sc-116447

B-Myb (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, which significantly enhances its transfection efficiency and protein expression capabilities. This lysate is enriched with human B-Myb, a member of the Myb family of transcription factors that plays a critical role in cell cycle regulation, differentiation, and apoptosis. B-Myb is particularly important during the G1/S transition and is involved in regulating the expression of genes required for DNA replication and cell cycle progression. In research, B-Myb (h): 293T Lysate is utilized to study the mechanisms of transcriptional regulation and the role of B-Myb in cell cycle control. Researchers employ this lysate in Western blotting to detect and quantify B-Myb expression levels under various experimental conditions, providing insights into its regulation and post-translational modifications. The lysate is also used in co-immunoprecipitation assays to identify protein-protein interactions involving B-Myb, which helps explain its role in transcriptional complexes and regulatory networks. Additionally, B-Myb (h): 293T Lysate is valuable in reporter assays to measure the transcriptional activity of B-Myb on specific target gene promoters. These assays help researchers understand how B-Myb influences gene expression during the cell cycle. The use of B-Myb (h): 293T Lysate allows for detailed investigations into the molecular mechanisms underlying cell cycle regulation and transcriptional control, contributing valuable insights into fundamental biological processes.