B-Myb Antibody (MYBAD10A) recommended for detection of B-Myb of human origin by WB, IP and IF

B-Myb Antibody (MYBAD10A): sc-81192

B-Myb Antibody (MYBAD10A) is rated 4.0 out of 5 by 4.
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Datasheets

    • B-Myb Antibody (MYBAD10A) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of B-Myb of human origin
    • recommended for detection of B-Myb of human origin by WB, IP and IF
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I am using B-Myb (MYBAD10A): sc-81192 for immunofluorescence, what dilution of this antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Robust signal in WB in human recombinant ZnT Robust signal in WB in human recombinant ZnT-9 fusion protein. -SCBT QC
Date published: 2015-04-17
Rated 4 out of 5 by from Positive signal in WB in human transfected Positive signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-28
Rated 4 out of 5 by from Strong band in WB in NIH3T3 whole cell lysate Strong band in WB in NIH3T3 whole cell lysate. -SCBT QC
Date published: 2015-02-13
Rated 4 out of 5 by from ChIP publishable data with mouse ESCs ChIP publishable data with mouse ESCs - SCBT Publication Review
Date published: 2014-02-27
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