Avidin Antibody (A4D12): sc-51761

Avidin Antibody (A4D12) is a mouse monoclonal IgG1 antibody that detects Avidin protein of avian origin by western blotting (WB) and immunofluorescence (IF). Avidin is a tetrameric glycoprotein predominantly found in egg white, renowned for its exceptional ability to bind Biotin with an extraordinarily high affinity, characterized by a dissociation constant (Kd) of approximately 10^-15 M^-1, which is the strongest known interaction between any ligand and a protein. This remarkable binding capability is crucial as Avidin plays a significant role in the nutritional absorption of Biotin; when Avidin binds to Biotin, forming a complex too large to be absorbed in the gastrointestinal tract, effectively preventing Biotin absorption from Biotin-deficient diets, particularly those rich in egg white. Avidin′s structure, consisting of four identical subunits, allows binding of four Biotin molecules, thereby enhancing capacity to inhibit Biotin absorption. Additionally, Avidin′s high solubility in water or salt solutions at physiological pH further underscores utility in various biochemical applications, making anti-Avidin antibody (A4D12) an essential tool for researchers studying Biotin interactions and related metabolic pathways in avian species.