Date published: 2025-12-24

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Aut7 Antibody (E-7): sc-133202

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Datasheets
  • Aut7 Antibody (E-7) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-117 representing full length Aut7 of Saccharomyces cerevisiae origin
  • recommended for detection of Aut7 of y origin by WB, IP, IF and ELISA
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for Aut7 Antibody (E-7) for WB applications. This reagent is now offered in a bundle with Aut7 Antibody (E-7) (see ordering information below).

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    Aut7 Antibody (E-7) is a mouse monoclonal IgG2b antibody that detects Aut7 in yeast by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Aut7 antibody (E-7) plays a crucial role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Aut7 (E-7) antibody recognizes a protein primarily located in the cytoplasm and involved in the cytoplasm-to-vacuole targeting (Cvt) pathway, a specialized form of autophagy that facilitates the selective transport of cytoplasmic components to the vacuole for degradation and recycling. This localization is significant because Aut7 maintains cellular integrity and responds to nutrient deprivation, particularly during nitrogen starvation, when Aut7 expression increases. Through facilitating the delivery of cytoplasmic materials to the vacuole, Aut7 ensures efficient resource management and elimination of potentially harmful components, contributing to overall cellular health and function.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Aut7 Antibody (E-7)

    sc-133202
    200 µg/ml
    $316.00

    Aut7 Antibody (E-7): m-IgG2b BP-HRP Bundle

    sc-548487
    200 µg Ab; 10 µg BP
    $354.00

    What is the recommended starting dilution for Western blot for sc-133202: Aut7 (E-7) monoclonal antibody?

    Asked by: jerojero
    The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-22
    • y_2025, m_12, d_21, h_4CST
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    Rated 5 out of 5 by from Good Western blot data of yeast recombinantGood Western blot data of yeast recombinant Aut7 fusion protein. -SCBT QC
    Date published: 2014-08-07
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    Aut7 Antibody (E-7) is rated 5.0 out of 5 by 1.
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