Date published: 2025-12-24

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Aut7 Antibody (B-2): sc-136973

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Datasheets
  • Aut7 Antibody (B-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1-117 representing full length Aut7 of Saccharomyces cerevisiae origin
  • recommended for detection of Aut7 of y origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Aut7 Antibody (B-2) for WB applications. This reagent is now offered in a bundle with Aut7 Antibody (B-2) (see ordering information below).

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    Aut7 Antibody (B-2) is a mouse monoclonal IgG1 antibody that detects Aut7 in yeast by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Aut7 antibody (B-2) plays a crucial role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Aut7 (B-2) antibody recognizes a protein primarily located in the cytoplasm, where Aut7 is involved in the cytoplasm-to-vacuole targeting (Cvt) pathway, a specialized form of autophagy that transports cytoplasmic components to the vacuole for degradation and recycling. This localization is vital because Aut7 ensures cellular components are efficiently processed and enables cells to respond to nutrient deprivation or stress conditions. Furthermore, proper functioning of Aut7 is critical for maintaining cellular health, as Aut7 helps prevent the accumulation of damaged proteins and organelles, thereby contributing to the overall metabolic balance within cells. Anti-Aut7 antibody (B-2) serves as an invaluable tool for researchers studying autophagy and its implications in various biological processes and diseases.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Aut7 Antibody (B-2)

    sc-136973
    200 µg/ml
    $316.00

    Aut7 Antibody (B-2): m-IgG Fc BP-HRP Bundle

    sc-539910
    200 µg Ab; 10 µg BP
    $354.00

    What blocking solution should I use with Aut7 (B-2): sc-136973 monoclonal antibody for Western blot?

    Asked by: jenniferc
    Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Great Western blot data of yeast recombinantGreat Western blot data of yeast recombinant Aut7 fusion protein. -SCBT QC
    Date published: 2014-07-31
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    Aut7 Antibody (B-2) is rated 5.0 out of 5 by 1.
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