ATRIP Antibody (F-7): sc-365383
- ATRIP Antibody (F-7) is a mouse monoclonal IgG2a κ ATRIP antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 492-791 mapping at the C-terminus of ATR-Interacting Protein of human origin
- ATRIP Antibody (F-7) is recommended for detection of all ATRIP isoforms of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ATRIP Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ATRIP Antibody (F-7) for WB applications. These reagents are now offered in bundles with ATRIP Antibody (F-7) (see ordering information below).
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ATRIP Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects ATRIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ATRIP antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATRIP plays a crucial role in DNA damage response, particularly in activating the ATR (ATM and Rad3-related) signaling pathway, which maintains genomic stability. ATRIP (F-7) antibody recognizes a protein involved in recruiting ATR to DNA damage sites, where ATRIP helps activate downstream checkpoint responses that prevent cell cycle progression until damage repair. This function ensures cells don′t replicate damaged DNA, which could lead to mutations and cancer. ATRIP interacts with replication protein A (RPA) complex, facilitating ATR binding to single-stranded DNA, enhancing cellular response to replication stress. Nuclear localization of ATRIP underscores its importance in DNA repair processes, making ATRIP a significant target for cancer biology studies and therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ATRIP Antibody (F-7) References:
- ATR and ATRIP: partners in checkpoint signaling. | Cortez, D., et al. 2001. Science. 294: 1713-6. PMID: 11721054
- Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. | Zou, L. and Elledge, SJ. 2003. Science. 300: 1542-8. PMID: 12791985
- ATRIP oligomerization is required for ATR-dependent checkpoint signaling. | Ball, HL. and Cortez, D. 2005. J Biol Chem. 280: 31390-6. PMID: 16027118
- Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks. | Itakura, E., et al. 2005. Mol Biol Cell. 16: 5551-62. PMID: 16176973
- Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires binding of ATRIP to ATR but not the stable DNA-binding or coiled-coil domains of ATRIP. | Kim, SM., et al. 2005. J Biol Chem. 280: 38355-64. PMID: 16186122
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATRIP Antibody (F-7) | sc-365383 | 200 µg/ml | $322.00 | |||
ATRIP Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-525878 | 200 µg Ab; 10 µg BP | $361.00 | |||
ATRIP Antibody (F-7): m-IgG2a BP-HRP Bundle | sc-546450 | 200 µg Ab; 10 µg BP | $361.00 | |||
ATRIP Antibody (F-7) AC | sc-365383 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ATRIP Antibody (F-7) HRP | sc-365383 HRP | 200 µg/ml | $322.00 | |||
ATRIP Antibody (F-7) FITC | sc-365383 FITC | 200 µg/ml | $336.00 | |||
ATRIP Antibody (F-7) PE | sc-365383 PE | 200 µg/ml | $349.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 488 | sc-365383 AF488 | 200 µg/ml | $364.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 546 | sc-365383 AF546 | 200 µg/ml | $364.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 594 | sc-365383 AF594 | 200 µg/ml | $364.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 647 | sc-365383 AF647 | 200 µg/ml | $364.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 680 | sc-365383 AF680 | 200 µg/ml | $364.00 | |||
ATRIP Antibody (F-7) Alexa Fluor® 790 | sc-365383 AF790 | 200 µg/ml | $364.00 |