Date published: 2025-9-5
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ATP7A Antibody (D-9) - Western Blotting - Image 397472
ATP7A 抗体 (D-9) Alexa Fluor® 488: sc-376467 AF488. Hep G2 (A) 和 SH-SY5Y (B) 全细胞裂解液中 ATP7A 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
ATP7A 抗体 (D-9) Alexa Fluor® 488: sc-376467 AF488. Hep G2 (A) 和 SH-SY5Y (B) 全细胞裂解液中 ATP7A 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

ATP7A 抗体 (D-9): sc-376467

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说明书
  • ATP7A 抗体 D-9 是小鼠单克隆 IgG2a κ，ATP7A抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-180的抗原，在human起源的ATP7A的N-terminal细胞质域内
  • ATP7A 抗体 (D-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ATP7A isoforms 1, 2, 4 and 5
  • 抗ATP7A抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATP7A (D-9): sc-376467的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是ATP7A Antibody (D-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与ATP7A Antibody (D-9) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外ATP7抗体，包括ATP5H

ATP7A抗体（D-9）是一种IgG2a κ小鼠单克隆ATP7A抗体（也被称为ATP7A抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测人类来源的ATP7A蛋白。ATP7A抗体（D-9）既有非偶联形式的抗ATP7A抗体，也有多种偶联形式的抗ATP7A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。铜外排转运体ATP7A和ATP7B将细胞内铜隔离到囊泡分泌途径中，以便从细胞中排出。ATP7A（也称为铜转运ATP酶1）作为一种跨膜铜转运P型ATP酶，在肠道系统铜吸收和肾脏铜再吸收中起着至关重要的作用。极化上皮细胞，如Madin-Darby犬肾细胞，是体内系统铜吸收和再吸收的生理学相关模型。尽管在大多数正常组织中检测不到ATP7A，但它在许多常见肿瘤类型的相当一部分中都有表达。ATP7A表达增加会使细胞对顺铂和卡铂产生抗性。ATP7A基因的突变会导致Menkes病，这是一种在幼儿期致命的疾病。ATP7B基因的突变会导致常染色体隐性遗传病--Wilson病，其特征是神经症状和肝损伤。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATP7A 抗体 (D-9) 参考文献:

  1. 铂类药物治疗期间铜转运体 ATP7A 表达的增加与卵巢癌患者的生存率低下有关。  |  Samimi, G., et al. 2003. Clin Cancer Res. 9: 5853-9. PMID: 14676106
  2. 人类铜转运 ATP 酶 ATP7B 核苷酸结合域的独特功能特性：对威尔逊病突变 E1064A, H1069Q, R1151H 和 C1104F 的分析。  |  Morgan, CT., et al. 2004. J Biol Chem. 279: 36363-71. PMID: 15205462
  3. 铜输出体 ATP7A 和 ATP7B 对顺铂及其类似物的细胞药理学的调节。  |  Samimi, G., et al. 2004. Mol Pharmacol. 66: 25-32. PMID: 15213293
  4. 在极化的 MDCK 细胞中调节门克思（MNK；ATP7A）铜转运 P 型 ATP 酶迁移的信号。  |  Greenough, M., et al. 2004. Am J Physiol Cell Physiol. 287: C1463-71. PMID: 15269005
  5. 通过酵母双杂交分析研究依赖铜的蛋白质与蛋白质之间的相互作用。  |  van Dongen, EM., et al. 2004. Biochem Biophys Res Commun. 323: 789-95. PMID: 15381069
  6. 人类铜转运体 Ctr1 在顺铂敏感和顺铂耐药细胞中转运铂类抗肿瘤药物中的作用。  |  Song, IS., et al. 2004. Mol Cancer Ther. 3: 1543-9. PMID: 15634647
  7. 铜转运 ATP 酶, 孟克氏症蛋白和威尔森氏症蛋白在成人和发育中的小脑中发挥着不同的作用。  |  Barnes, N., et al. 2005. J Biol Chem. 280: 9640-5. PMID: 15634671
  8. ATP7A相关铜转运疾病--新兴概念和未来趋势。  |  Kaler, SG. 2011. Nat Rev Neurol. 7: 15-29. PMID: 21221114
  9. 少量的功能性ATP7A蛋白允许轻度表型。  |  Møller, LB. 2015. J Trace Elem Med Biol. 31: 173-7. PMID: 25172213
  10. Menkes病之谜中ATP7A调节的酶金属化和转运。  |  Horn, N. and Wittung-Stafshede, P. 2021. Biomedicines. 9: PMID: 33917579
  11. Elesclomol通过降解ATP7A诱导结肠直肠癌细胞发生铜依赖性铁死亡。  |  Gao, W., et al. 2021. Mol Oncol. 15: 3527-3544. PMID: 34390123
  12. ATP7A相关的铜转运障碍：临床亚型的系统回顾和定义。  |  De Feyter, S., et al. 2023. J Inherit Metab Dis. 46: 163-173. PMID: 36692329

订购信息

产品名称产品编号规格价格数量收藏夹

ATP7A 抗体 (D-9)

sc-376467
200 µg/ml
$316.00

ATP7A (D-9): m-IgGκ BP-HRP 套装

sc-552045
200 µg Ab; 40 µg BP
$354.00

ATP7A 抗体 (D-9) AC

sc-376467 AC
500 µg/ml, 25% agarose
$416.00

ATP7A 抗体 (D-9) HRP

sc-376467 HRP
200 µg/ml
$316.00

ATP7A 抗体 (D-9) FITC

sc-376467 FITC
200 µg/ml
$330.00

ATP7A 抗体 (D-9) PE

sc-376467 PE
200 µg/ml
$343.00

ATP7A 抗体 (D-9) Alexa Fluor® 488

sc-376467 AF488
200 µg/ml
$357.00

ATP7A 抗体 (D-9) Alexa Fluor® 546

sc-376467 AF546
200 µg/ml
$357.00

ATP7A 抗体 (D-9) Alexa Fluor® 594

sc-376467 AF594
200 µg/ml
$357.00

ATP7A 抗体 (D-9) Alexa Fluor® 647

sc-376467 AF647
200 µg/ml
$357.00

ATP7A 抗体 (D-9) Alexa Fluor® 680

sc-376467 AF680
200 µg/ml
$357.00

ATP7A 抗体 (D-9) Alexa Fluor® 790

sc-376467 AF790
200 µg/ml
$357.00

Does this antibody also have a peptide that can be purchased against it to ensure antibody specificity? Thanks.

Asked by: kschoon1
Thank you for your question. This mouse monoclonal, ATP7A Antibody (D-9): sc-376467, is not raised against a peptide so no blocking peptide is available. The antibody is raised against a recombinant protein corresponding to amino acids 1-180 mapping within an N-terminal cytoplasmic domain of ATP7A of human origin. Because of the length of this protein, an antibody blocking or neutralization experiment would not be efficient. We would recommend performing alternative controls to evaluate the specificity of the antibody, for example using RNAi or CRISPR to silence or knockout the target gene.
Answered by: Technical Support
Date published: 2017-02-18

What is the recommended starting dilution for immunostaining with ATP7A (D-9): sc-376467 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for immunostaining is 1:50 with a dilution range of 1:50-1:500. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 9
Date published: 2017-01-20
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Rated 5 out of 5 by from This antibody's function very well.Wb used rat protein with a dilution of 1:500,the band is special but not so clear,I will tried with a dilution of 1:200 to try again
Date published: 2022-11-12
Rated 3 out of 5 by from Okay antibodyThis is an okay antibody, weak detection but visible at high contrast.
Date published: 2021-11-22
Rated 5 out of 5 by from Really goodReally good and clean signal on WB. Dilution 1:1000.
Date published: 2019-11-27
Rated 5 out of 5 by from great antibodyUsing 1:500 dilution I could make it work in bladder carcinoma cells.
Date published: 2017-09-10
Rated 5 out of 5 by from SuperbGreat western blot data; also nice IHC staining data
Date published: 2017-02-04
Rated 5 out of 5 by from Best bang for your buck!This is a very strong antibody that will give you nice solid bands in your Western blot. Reliable and consistent, you can't go wrong!
Date published: 2016-12-21
Rated 4 out of 5 by from I had to use 1:500 dilution in order to makeI had to use 1:500 dilution in order to make it work. In the end it worked better with cell samples rather than tissue.
Date published: 2015-09-10
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-03
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ATP7A 抗体 (D-9) is rated 4.6 out of 5 by 8.
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