Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATP-citrate synthase CRISPR/Cas9 KO Plasmid (m): sc-430516
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP-citrate synthase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ATP-citrate synthase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP-citrate synthase Antibody (5F8D11): sc-517267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP-citrate synthase CRISPR/Cas9 KO Plasmid (m) | sc-430516 | 20 µg | $397.00 |
Overview
Mouse Acly encodes ATP-citrate synthase (ACLY), a cytosolic enzyme that converts citrate and CoA into acetyl‑CoA and oxaloacetate, linking mitochondrial carbon flux to cytoplasmic metabolism. By supplying acetyl‑CoA for de novo fatty acid and cholesterol synthesis, ACLY supports lipogenesis and membrane biogenesis, and it also contributes to acetyl‑CoA pools used for protein and histone acetylation. This activity integrates nutrient sensing with pathways controlling glycolysis–TCA cycle coupling, lipid homeostasis, and epigenetic regulation of gene expression. Dysregulated ACLY-dependent acetyl‑CoA production has been associated with altered metabolic remodeling, inflammatory signaling, and proliferation-related phenotypes in disease-relevant models.
ATP-citrate synthase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Acly gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Acly together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Acly open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ATP-citrate synthase protein expression.
This CRISPR knockout system enables efficient generation of Acly-deficient cell models for investigation of ATP-citrate synthase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Acly exon(s) critical for ATP-citrate synthase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Acly genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ATP-citrate synthase CRISPR/Cas9 KO Plasmid (m) and ATP-citrate synthase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Acly locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ATP-citrate synthase HDR Plasmid (m) and ATP-citrate synthase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Acly homology arms to support homology-directed repair at defined Acly target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.