ATGL Antibody (F-7): sc-365278

ATGL Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ATGL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ATGL antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ATGL, also known as adipose triglyceride lipase or patatin-like phospholipase domain-containing protein 2 (PNPLA2), plays a crucial role in lipid metabolism by catalyzing the hydrolysis of stored triglycerides in adipose tissue, thus facilitating the mobilization of fatty acids for energy production. This process is particularly important in energy homeostasis and metabolic regulation, as ATGL activity is tightly regulated by hormonal signals, such as insulin, which inversely modulates the expression of ATGL and adiponutrin (ADPN). ATGL consists of 486 amino acids and features a highly conserved N-terminal patatin domain, which is essential for lipase activity, along with a glycine-rich region and specific active site motifs that are critical for enzymatic function. Understanding ATGL dynamics and regulatory mechanisms is vital for elucidating the pathophysiology of metabolic disorders, including obesity and diabetes, where dysregulation of lipid metabolism is a key factor.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ATGL Antibody (F-7) References:

1. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase.  |  Zimmermann, R., et al. 2004. Science. 306: 1383-6. PMID: 15550674
2. Expression, regulation, and triglyceride hydrolase activity of Adiponutrin family members.  |  Lake, AC., et al. 2005. J Lipid Res. 46: 2477-87. PMID: 16150821
3. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-α and PGC-1.  |  Haemmerle, G., et al. 2011. Nat Med. 17: 1076-85. PMID: 21857651
4. ATGL Promotes Autophagy/Lipophagy via SIRT1 to Control Hepatic Lipid Droplet Catabolism.  |  Sathyanarayan, A., et al. 2017. Cell Rep. 19: 1-9. PMID: 28380348
5. ATGL-dependent white adipose tissue lipolysis controls hepatocyte PPARα activity.  |  Fougerat, A., et al. 2022. Cell Rep. 39: 110910. PMID: 35675775
6. ATGL is a biosynthetic enzyme for fatty acid esters of hydroxy fatty acids.  |  Patel, R., et al. 2022. Nature. 606: 968-975. PMID: 35676490
7. Blocking Lipid Uptake Pathways Does not Prevent Toxicity in Adipose Triglyceride Lipase (ATGL) Deficiency.  |  Oluwadare, J., et al. 2022. J Lipid Res. 63: 100274. PMID: 36115595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATGL Antibody (F-7) has 44 citations in a variety of scientific publications.

Primary antibodies like Anti-ATGL Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATGL Antibody (F-7) Specifications

Alternate Product Name: ATGL

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 361-504

Epitope Target: ATGL

Epitope Species: human

Product Reactivity/Detection: ATGL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for ATGL Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin