Atg4a Antibody (1458CT808.66.25.69): sc-517309

Atg4a Antibody (1458CT808.66.25.69) is a mouse monoclonal IgG2b kappa light chain antibody that detects Atg4a protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Atg4a antibody (1458CT808.66.25.69) is available as a non-conjugated format. Atg4a, also known as ATG4 autophagy related 4 homolog A or APG4A, is a crucial cysteine protease consisting of 398 amino acids that plays a vital role in autophagy, essential for cellular homeostasis and degradation of damaged organelles and proteins. Located primarily in the cytoplasm, Atg4a (1458CT808.66.25.69) monoclonal antibody targets a protein responsible for cleaving the C-terminal part of specific proteins, including GABARAP and MAP1LC3, facilitating autophagosome formation - double-membrane vesicles that engulf cellular debris for lysosomal degradation. Proper functioning of Atg4a regulates autophagy and influences cellular responses to stress and nutrient deprivation. Multiple alternatively spliced isoforms of Atg4a exist, and N-ethylmaleimide can inhibit Atg4a activity, highlighting post-translational modifications in regulatory mechanisms. Anti-Atg4a antibody (1458CT808.66.25.69) serves as an invaluable tool for researchers studying autophagy and implications in various diseases, including neurodegenerative disorders and cancer.

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Atg4a Antibody (1458CT808.66.25.69) References:

1. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.  |  Mariño, G., et al. 2003. J Biol Chem. 278: 3671-8. PMID: 12446702
2. The COOH terminus of GATE-16, an intra-Golgi transport modulator, is cleaved by the human cysteine protease HsApg4A.  |  Scherz-Shouval, R., et al. 2003. J Biol Chem. 278: 14053-8. PMID: 12473658
3. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.  |  Kabeya, Y., et al. 2004. J Cell Sci. 117: 2805-12. PMID: 15169837
4. Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3.  |  Tanida, I., et al. 2006. FEBS J. 273: 2553-62. PMID: 16704426
5. Reactive oxygen species are essential for autophagy and specifically regulate the activity of Atg4.  |  Scherz-Shouval, R., et al. 2007. EMBO J. 26: 1749-60. PMID: 17347651