ATF-6α Antibody (F-7): sc-166659

ATF-6α Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody designed to specifically target the activating transcription factor 6 alpha, also known as ATF-6α. ATF-6α monoclonal antibody (F-7) recognizes the epitope spanning amino acids 31-310 of the human ATF-6 protein and is validated for detecting ATF-6α in mouse, rat, and human samples using a variety of techniques including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ATF-6α is a crucial member of the basic leucine zipper (bZIP) family of transcription factors that plays a vital role in the cellular response to endoplasmic reticulum (ER) stress. Under conditions of ER stress, ATF-6α undergoes proteolytic cleavage, allowing its active form to translocate to the nucleus where ATF-6α regulates the expression of ER chaperones and other genes essential for maintaining protein homeostasis and cell survival. This function is critical for understanding mechanisms related to protein folding diseases, cancer, and other stress-related pathologies. ATF-6α exists in cells as either an ATF-6β homodimer or an ATF-6α/β heterodimer, both of which bind to the nuclear transcription factor Y C (NF-YC) to induce the transcription of ER chaperone proteins. ATF-6α (F-7) antibody is available in both non-conjugated form and a range of conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options for diverse experimental applications.

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ATF-6α Antibody (F-7) References:

1. Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic reticulum stress response.  |  Wang, Y., et al. 2000. J Biol Chem. 275: 27013-20. PMID: 10856300
2. ATF6 as a transcription activator of the endoplasmic reticulum stress element: thapsigargin stress-induced changes and synergistic interactions with NF-Y and YY1.  |  Li, M., et al. 2000. Mol Cell Biol. 20: 5096-106. PMID: 10866666
3. ATF6 activated by proteolysis binds in the presence of NF-Y (CBF) directly to the cis-acting element responsible for the mammalian unfolded protein response.  |  Yoshida, H., et al. 2000. Mol Cell Biol. 20: 6755-67. PMID: 10958673
4. IRE1 and efferent signaling from the endoplasmic reticulum.  |  Urano, F., et al. 2000. J Cell Sci. 113 Pt 21: 3697-702. PMID: 11034898
5. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
6. Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation.  |  Bu, LJ., et al. 2017. World J Gastroenterol. 23: 986-998. PMID: 28246472
7. Atf-6 Regulates Lifespan through ER-Mitochondrial Calcium Homeostasis.  |  Burkewitz, K., et al. 2020. Cell Rep. 32: 108125. PMID: 32905769
8. Cardiac hypertrophy caused by hyperthyroidism in rats: the role of ATF-6 and TRPC1 channels.  |  Bektur Aykanat, NE., et al. 2021. Can J Physiol Pharmacol. 99: 1226-1233. PMID: 34283935
9. Dual role of endoplasmic reticulum stress-ATF-6 activation in autophagy and apoptosis induced by cyclic stretch in myoblast.  |  Zhang, Q., et al. 2023. Apoptosis. 28: 796-809. PMID: 36881290
10. Correction to: dual role of endoplasmic reticulum stress-ATF-6 activation in autophagy and apoptosis induced by cyclic stretch in myoblast.  |  Zhang, Q., et al. 2023. Apoptosis. 28: 1501. PMID: 37439923
11. Activating transcription factor (ATF) 6 upregulates cystathionine β synthetase (CBS) expression and hydrogen sulfide (H2S) synthesis to ameliorate liver metabolic damage.  |  Dong, B., et al. 2023. Eur J Med Res. 28: 540. PMID: 38007457
12. Interaction of ATF6 and serum response factor.  |  Zhu, C., et al. 1997. Mol Cell Biol. 17: 4957-66. PMID: 9271374

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATF-6α Antibody (F-7) has 166 citations in a variety of scientific publications.

Primary antibodies like Anti-ATF-6α Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATF-6α Antibody (F-7) Specifications

Alternate Product Name: ATF-6α

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-310

Epitope Target: ATF-6

Epitope Species: human

Product Reactivity/Detection: ATF-6α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 166 citations in various scientific publications

Available Conjugates/Forms for ATF-6α Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent