ATF-6α Double Nickase Plasmid (m): sc-432646-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATF-6α Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATF-6α Double Nickase Plasmid (m) and ATF-6α Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Atf6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATF-6α Antibody (F-7): sc-166659
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATF-6α Double Nickase Plasmid (m) | sc-432646-NIC | 20 µg | $410.00 | |||
ATF-6α Double Nickase Plasmid (m2) | sc-432646-NIC-2 | 20 µg | $410.00 |
Activating transcription factor 6 (Atf6) encodes ATF-6α, an ER-resident transmembrane bZIP transcription factor that serves as a principal sensor of unfolded protein accumulation. During ER stress, ATF-6α is transported to the Golgi for regulated intramembrane proteolysis, releasing a nuclear fragment that induces transcriptional programs controlling proteostasis, including ER chaperones, ER-associated degradation components, and lipid metabolic genes. ATF-6α functions within the unfolded protein response alongside IRE1/XBP1 and PERK/eIF2α signaling to restore ER homeostasis and coordinate adaptive remodeling. Dysregulated ATF6 activity has been linked to pathobiology associated with chronic ER stress, including metabolic dysfunction, neurodegenerative processes, and cardiovascular phenotypes, making Atf6 a widely used node for mechanistic studies of stress signaling.
ATF-6α Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Atf6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Atf6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Atf6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Atf6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.