ATF-5 CRISPR/Cas9 KO Plasmid (m): sc-430721
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATF-5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ATF-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATF-5 Antibody (E-10): sc-377168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATF-5 CRISPR/Cas9 KO Plasmid (m) | sc-430721 | 20 µg | $397.00 |
Overview
Activating transcription factor 5 (ATF-5), encoded by the mouse Atf5 gene, is a basic leucine zipper (bZIP) transcription factor that regulates gene expression programs controlling cellular stress adaptation, differentiation, and survival. ATF-5 participates in integrated stress response signaling and can couple upstream eIF2α/ATF4-associated cues to transcriptional outputs that influence mitochondrial function and proteostasis. In the nervous system and other developing tissues, ATF-5 has been linked to lineage decisions and maintenance of proliferative or progenitor-like states through context-dependent transcriptional control. Dysregulated ATF-5 activity has been associated with aberrant stress tolerance and altered cell survival pathways, supporting its relevance for mechanistic studies of disease-related remodeling of transcriptional networks.
ATF-5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Atf5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Atf5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Atf5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ATF-5 protein expression.
This CRISPR knockout system enables efficient generation of Atf5-deficient cell models for investigation of ATF-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Atf5 exon(s) critical for ATF-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Atf5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ATF-5 CRISPR/Cas9 KO Plasmid (m) and ATF-5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Atf5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ATF-5 HDR Plasmid (m) and ATF-5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Atf5 homology arms to support homology-directed repair at defined Atf5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.