AspRS Antibody (D-4): sc-166534

AspRS Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects AspRS (mitochondrial aspartyl-tRNA synthetase) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). AspRS is a crucial 645 amino acid protein belonging to the class-II aminoacyl-tRNA synthetase family, primarily localized in the mitochondrial matrix where AspRS functions as a homodimer to aminoacylate tRNA, a vital process for protein synthesis. AspRS contains a 47 amino acid mitochondrial targeting signal, which is essential for proper localization and function within the mitochondria, as well as several conserved residues that are critical for ATP binding, tRNA binding, and the recognition of aspartic acid. The gene encoding AspRS is located on chromosome 1q25.1, and mutations in this gene can lead to leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation (LBSL), an autosomal recessive disorder characterized by symptoms such as cerebellar ataxia, spasticity, and dorsal column dysfunction, which may also include mild cognitive deficits. Anti-AspRS antibody (D-4) is an invaluable tool for researchers studying mitochondrial function and related pathologies, providing insights into the molecular mechanisms underlying these critical biological processes.

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AspRS Antibody (D-4) References:

1. Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.  |  Bonnefond, L., et al. 2005. Biochemistry. 44: 4805-16. PMID: 15779907
2. The nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori: anticodon-binding domain mutations that impact tRNA specificity and heterologous toxicity.  |  Chuawong, P. and Hendrickson, TL. 2006. Biochemistry. 45: 8079-87. PMID: 16800632
3. Peculiar inhibition of human mitochondrial aspartyl-tRNA synthetase by adenylate analogs.  |  Messmer, M., et al. 2009. Biochimie. 91: 596-603. PMID: 19254750
4. Architecture and metamorphosis.  |  Guo, M. and Yang, XL. 2014. Top Curr Chem. 344: 89-118. PMID: 23536245
5. Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori.  |  Songsiriritthigul, C., et al. 2017. Acta Crystallogr F Struct Biol Commun. 73: 62-69. PMID: 28177315
6. Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations.  |  González-Serrano, LE., et al. 2018. J Biol Chem. 293: 13604-13615. PMID: 30006346
7. Global, regional, and national burden of infertility attributable to PCOS, 1990-2019.  |  Liu, X., et al. 2024. Hum Reprod. 39: 108-118. PMID: 38011904
8. Global and regional trends in the incidence and prevalence of uterine fibroids and attributable risk factors at the national level from 2010 to 2019: A worldwide database study.  |  Dai, Y., et al. 2024. Chin Med J (Engl). 137: 2583-2589. PMID: 38407293
9. Global, regional, and national burden of stroke from 1990 to 2019: A temporal trend analysis based on the Global Burden of Disease Study 2019.  |  Zhang, L., et al. 2024. Int J Stroke. 19: 686-694. PMID: 38567822
10. Prevalence of cardiovascular events in a population-based registry of patients with systemic lupus erythematosus.  |  Joyce, DP., et al. 2024. Arthritis Res Ther. 26: 160. PMID: 39272198

The preferred AspRS antibody is AspRS Antibody (C-6): sc-166535.