ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 CRISPR/Cas9 KO Plasmid (h): sc-400429

Overview

ASGR1 encodes asialoglycoprotein receptor 1 (ASGPR1), a hepatocyte-enriched, Ca²⁺-dependent lectin that forms the asialoglycoprotein receptor complex on the plasma membrane and mediates clathrin-dependent endocytosis of desialylated glycoproteins. By binding terminal galactose or N-acetylgalactosamine residues, ASGPR1 contributes to hepatic clearance of circulating glycoproteins and influences receptor recycling and lysosomal trafficking pathways. This receptor-ligand system is widely used as a model for studying liver-specific uptake mechanisms and glycoprotein quality control. Altered ASGPR1 expression or function has been examined in the context of metabolic and inflammatory liver states and is relevant to investigations of hepatocyte surface receptor regulation and endocytic capacity.

ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ASGR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ASGR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ASGR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 protein expression.

This CRISPR knockout system enables efficient generation of ASGR1-deficient cell models for investigation of ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ASGR1 exon(s) critical for ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ASGR1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 CRISPR/Cas9 KO Plasmid (h) and ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ASGR1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 HDR Plasmid (h) and ASGPR1/Asialoglycoprotein Receptor 1/ASGR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ASGR1 homology arms to support homology-directed repair at defined ASGR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.