ASCC1 CRISPR/Cas9 KO Plasmid (h): sc-417767
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ASCC1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ASCC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASCC1 CRISPR/Cas9 KO Plasmid (h) | sc-417767 | 20 µg | $397.00 |
Overview
ASCC1 (activating signal cointegrator 1 complex subunit 1) is a nuclear factor implicated in transcriptional regulation and genome maintenance, functioning within the ASC-1/ASCC coactivator complex that interfaces with RNA polymerase II–dependent gene expression. ASCC1 has been linked to cellular responses to genotoxic stress and coordination of DNA repair-associated processes, supporting proper cell-cycle progression and proteostasis under stress conditions. Disruption of ASCC1 function has been associated with neuromuscular disease phenotypes, consistent with an essential role in maintaining tissue homeostasis and gene expression programs in differentiated cells. As a result, ASCC1 is studied in pathways connecting transcription, DNA damage signaling, and stress-responsive regulatory networks.
ASCC1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ASCC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ASCC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ASCC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ASCC1 protein expression.
This CRISPR knockout system enables efficient generation of ASCC1-deficient cell models for investigation of ASCC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ASCC1 exon(s) critical for ASCC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ASCC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ASCC1 CRISPR/Cas9 KO Plasmid (h) and ASCC1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ASCC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ASCC1 HDR Plasmid (h) and ASCC1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ASCC1 homology arms to support homology-directed repair at defined ASCC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.