ASB-9 Antibody (E-12): sc-166723
- ASB-9 Antibody (E-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-70 mapping at the N-terminus of ASB-9 of human origin
- ASB-9 Antibody (E-12) is recommended for detection of ASB-9 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ASB-9 Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASB-9 Antibody (E-12) for WB and IHC(P) applications. These reagents are now offered in bundles with ASB-9 Antibody (E-12) (see ordering information below).
ASB-9 Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASB-9 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ASB-9 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASB-9 is a member of the ankyrin repeat and SOCS box-containing (ASB) protein family, which plays a crucial role in regulating various biological processes through its function as a ubiquitin ligase. Specifically, ASB-9 contains six ankyrin repeats and one SOCS box domain, allowing ASB-9 to interact with specific binding partners, such as creatine kinase-B, which ASB-9 targets for degradation. This interaction is vital for maintaining appropriate levels of creatine kinase-B within the cell, thereby influencing energy metabolism and cellular signaling pathways. Additionally, ASB-9 exists in two isoforms due to alternative splicing, which may further diversify ASB-9′s functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ASB-9 Antibody (E-12) References:
- Cloning and characterization of the genes encoding the ankyrin repeat and SOCS box-containing proteins Asb-1, Asb-2, Asb-3 and Asb-4. | Kile, BT., et al. 2000. Gene. 258: 31-41. PMID: 11111040
- The SOCS box: a tale of destruction and degradation. | Kile, BT., et al. 2002. Trends Biochem Sci. 27: 235-41. PMID: 12076535
- ASB proteins interact with Cullin5 and Rbx2 to form E3 ubiquitin ligase complexes. | Kohroki, J., et al. 2005. FEBS Lett. 579: 6796-802. PMID: 16325183
- Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. | Debrincat, MA., et al. 2007. J Biol Chem. 282: 4728-4737. PMID: 17148442
- Hundreds of ankyrin-like repeats in functionally diverse proteins: mobile modules that cross phyla horizontally? | Bork, P. 1993. Proteins. 17: 363-74. PMID: 8108379
- Twenty proteins containing a C-terminal SOCS box form five structural classes. | Hilton, DJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 114-9. PMID: 9419338
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASB-9 Antibody (E-12) | sc-166723 | 200 µg/ml | $322.00 | |||
ASB-9 Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-521723 | 200 µg Ab, 40 µg BP | $361.00 | |||
ASB-9 Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-533503 | 200 µg Ab; 20 µg BP | $361.00 | |||
ASB-9 Antibody (E-12) AC | sc-166723 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ASB-9 Antibody (E-12) HRP | sc-166723 HRP | 200 µg/ml | $322.00 | |||
ASB-9 Antibody (E-12) FITC | sc-166723 FITC | 200 µg/ml | $336.00 | |||
ASB-9 Antibody (E-12) PE | sc-166723 PE | 200 µg/ml | $349.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 488 | sc-166723 AF488 | 200 µg/ml | $364.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 546 | sc-166723 AF546 | 200 µg/ml | $364.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 594 | sc-166723 AF594 | 200 µg/ml | $364.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 647 | sc-166723 AF647 | 200 µg/ml | $364.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 680 | sc-166723 AF680 | 200 µg/ml | $364.00 | |||
ASB-9 Antibody (E-12) Alexa Fluor® 790 | sc-166723 AF790 | 200 µg/ml | $364.00 |