Artemis Antibody (C-8): sc-518193
- Artemis Antibody (C-8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 362-384 of Artemis of human origin
- recommended for detection of Artemis of human origin by WB, IP, IF and ELISA
- Anti-Artemis Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Artemis Antibody (C-8) for WB applications. This reagent is now offered in a bundle with Artemis Antibody (C-8) (see ordering information below).
QUICK LINKS
SEE ALSO...
Artemis Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Artemis of human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Artemis (C-8) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Artemis plays a crucial role in DNA damage response, specifically in repairing double-strand breaks, which are critical for maintaining genomic stability. Artemis (C-8) monoclonal antibody targets a protein primarily located in the nucleus, where Artemis interacts with DNA and other repair proteins, highlighting its importance in cellular processes that safeguard against mutations and cancer. Artemis functions as a single-strand-specific 5′ to 3′ exonuclease and forms a complex with DNA-dependent protein kinase (DNA-PKcs), which phosphorylates Artemis, enabling endonucleolytic activity on 5′ and 3′ overhangs and hairpins. These activities are vital for V(D)J recombination and DNA end processing during nonhomologous end joining, essential mechanisms for immune system development. Mutations in Artemis can lead to severe combined immunodeficiency disease (SCID), also known as "bubble boy" disease, characterized by a lack of B and T lymphocytes and increased sensitivity to DNA-damaging agents. The human Artemis gene is located on chromosome 10p13 and encodes a protein consisting of 577 amino acids, underscoring its significance in both normal cellular function and disease pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Artemis Antibody (C-8) References:
- Human DNA repair genes. | Wood, RD., et al. 2001. Science. 291: 1284-9. PMID: 11181991
- Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency. | Moshous, D., et al. 2001. Cell. 105: 177-86. PMID: 11336668
- Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. | Ma, Y., et al. 2002. Cell. 108: 781-94. PMID: 11955432
- Does artemis end the hunt for the hairpin-opening activity in V(D)J recombination? | Schlissel, MS. 2002. Cell. 109: 1-4. PMID: 11955439
- A founder mutation in Artemis, an SNM1-like protein, causes SCID in Athabascan-speaking Native Americans. | Li, L., et al. 2002. J Immunol. 168: 6323-9. PMID: 12055248
- The gene for severe combined immunodeficiency disease in Athabascan-speaking Native Americans is located on chromosome 10p. | Li, L., et al. 1998. Am J Hum Genet. 62: 136-44. PMID: 9443881
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Artemis Antibody (C-8) | sc-518193 | 200 µg/ml | $322.00 | |||
Artemis Antibody (C-8): m-IgG1 BP-HRP Bundle | sc-550933 | 200 µg Ab; 20 µg BP | $361.00 | |||
Artemis Antibody (C-8) AC | sc-518193 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Artemis Antibody (C-8) HRP | sc-518193 HRP | 200 µg/ml | $322.00 | |||
Artemis Antibody (C-8) FITC | sc-518193 FITC | 200 µg/ml | $336.00 | |||
Artemis Antibody (C-8) PE | sc-518193 PE | 200 µg/ml | $349.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 488 | sc-518193 AF488 | 200 µg/ml | $364.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 546 | sc-518193 AF546 | 200 µg/ml | $364.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 594 | sc-518193 AF594 | 200 µg/ml | $364.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 647 | sc-518193 AF647 | 200 µg/ml | $364.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 680 | sc-518193 AF680 | 200 µg/ml | $364.00 | |||
Artemis Antibody (C-8) Alexa Fluor® 790 | sc-518193 AF790 | 200 µg/ml | $364.00 |