Arp-T2 Antibody (H-2): sc-515601
- Arp-T2 Antibody (H-2) is a mouse monoclonal IgG1 κ Arp-T2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 126-145 within an internal region of Arp-T2 of mouse origin
- Arp-T2 Antibody (H-2) is recommended for detection of Arp-T2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Arp-T2 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Arp-T2 Antibody (H-2) for WB applications. These reagents are now offered in bundles with Arp-T2 Antibody (H-2) (see ordering information below).
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Arp-T2 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Arp-T2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Arp-T2 antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Arp-T2, also known as Actin-related protein T2 or Arp-M2, is a 377-amino acid structural cytoskeleton protein that plays a crucial role in the organization and dynamics of the cytoskeleton. Arp-T2 is particularly important due to involvement in the formation of the actin filament network, which is essential for various cellular processes such as cell shape maintenance, motility, and division. Arp-T2 contains an actin-like ATP/ADP-binding pocket and two nuclear export signals, which are vital for function and regulation within the cell. Notably, Arp-T2 shares 43% sequence identity with β-actin and possesses unique cysteine residues that differentiate Arp-T2 from other actin family members. With significant expression in the testis, Arp-T2 is believed to play a pivotal role in spermatid formation, highlighting importance in reproductive biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Arp-T2 Antibody (H-2) References:
- Actin-binding properties and colocalization with actin during spermiogenesis of mammalian sperm calicin. | Lécuyer, C., et al. 2000. Biol Reprod. 63: 1801-10. PMID: 11090452
- Identification of two cDNAs for human actin-related proteins (Arps) that have remarkable similarity to conventional actin. | Harata, M., et al. 2001. Biochim Biophys Acta. 1522: 130-3. PMID: 11750065
- Novel actin-related proteins Arp-T1 and Arp-T2 as components of the cytoskeletal calyx of the mammalian sperm head. | Heid, H., et al. 2002. Exp Cell Res. 279: 177-87. PMID: 12243744
- Characterization of boar sperm cytoskeletal cylicin II as an actin-binding protein. | Rousseaux-Prévost, R., et al. 2003. Biochem Biophys Res Commun. 303: 182-9. PMID: 12646184
- Nuclear localization of profilin III-ArpM1 complex in mouse spermiogenesis. | Hara, Y., et al. 2008. FEBS Lett. 582: 2998-3004. PMID: 18692047
- Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments. | LeClaire, LL., et al. 2008. J Cell Biol. 182: 647-54. PMID: 18725535
- Basic proteins of the perinuclear theca of mammalian spermatozoa and spermatids: a novel class of cytoskeletal elements. | Longo, FJ., et al. 1987. J Cell Biol. 105: 1105-20. PMID: 3308904
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arp-T2 Antibody (H-2) | sc-515601 | 200 µg/ml | $322.00 | |||
Arp-T2 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-531434 | 200 µg Ab; 10 µg BP | $361.00 | |||
Arp-T2 Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-525122 | 200 µg Ab, 40 µg BP | $361.00 | |||
Arp-T2 Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-544553 | 200 µg Ab; 20 µg BP | $361.00 | |||
Arp-T2 Antibody (H-2) AC | sc-515601 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Arp-T2 Antibody (H-2) HRP | sc-515601 HRP | 200 µg/ml | $322.00 | |||
Arp-T2 Antibody (H-2) FITC | sc-515601 FITC | 200 µg/ml | $336.00 | |||
Arp-T2 Antibody (H-2) PE | sc-515601 PE | 200 µg/ml | $349.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 488 | sc-515601 AF488 | 200 µg/ml | $364.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 546 | sc-515601 AF546 | 200 µg/ml | $364.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 594 | sc-515601 AF594 | 200 µg/ml | $364.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 647 | sc-515601 AF647 | 200 µg/ml | $364.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 680 | sc-515601 AF680 | 200 µg/ml | $364.00 | |||
Arp-T2 Antibody (H-2) Alexa Fluor® 790 | sc-515601 AF790 | 200 µg/ml | $364.00 | |||
Arp-T2 (H-2) Neutralizing Peptide | sc-515601 P | 100 µg/0.5 ml | $69.00 |