Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARP-1 CRISPR/Cas9 KO Plasmid (h): sc-405241
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARP-1 Antibody (E-11): sc-271940
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARP-1 CRISPR/Cas9 KO Plasmid (h) | sc-405241 | 20 µg | $397.00 |
Overview
NR2F2 encodes the human orphan nuclear receptor ARP-1 (also known as COUP-TFII), a ligand-independent transcription factor that binds DNA as a mono- or heterodimer to regulate developmental and metabolic gene programs. ARP-1 integrates signaling inputs that shape cell fate decisions, angiogenic and lymphangiogenic processes, and organogenesis through transcriptional control of networks linked to nuclear receptor crosstalk and enhancer activity. In adult tissues, NR2F2 influences vascular smooth muscle and endothelial biology, adipogenesis, and mitochondrial/metabolic homeostasis, with context-dependent effects on proliferation and differentiation. Dysregulated NR2F2 expression or activity has been associated with cancer biology, congenital cardiovascular defects, and metabolic disorders, making it a widely used node for mechanistic studies of transcriptional regulation and lineage specification.
ARP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NR2F2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NR2F2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NR2F2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARP-1 protein expression.
This CRISPR knockout system enables efficient generation of NR2F2-deficient cell models for investigation of ARP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NR2F2 exon(s) critical for ARP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NR2F2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARP-1 CRISPR/Cas9 KO Plasmid (h) and ARP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NR2F2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARP-1 HDR Plasmid (h) and ARP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NR2F2 homology arms to support homology-directed repair at defined NR2F2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.