Arnt 1 Antibody (4G9): sc-65639

Arnt 1 Antibody (4G9) is a mouse monoclonal IgG1 antibody that recognizes Arnt 1 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Arnt 1, also known as aryl hydrocarbon receptor nuclear translocator, plays a crucial role in cellular signaling by facilitating the transcriptional activity of the aryl hydrocarbon receptor (AhR). Arnt 1 (4G9) antibody targets a protein primarily located in the nucleus, where Arnt 1 forms heterodimers with AhR upon ligand binding, allowing for the regulation of genes involved in xenobiotic metabolism and cellular responses to environmental toxins. Nuclear localization of Arnt 1 ensures that transcriptional regulation occurs at the site of gene expression, thereby influencing various biological processes, including cell proliferation, differentiation, and response to stress. Arnt 1 also regulates hypoxia-inducible factors, linking oxygen availability to gene expression, which is essential for maintaining cellular homeostasis. With significant roles in mediating environmental pollutant effects and involvement in critical cellular pathways, anti-Arnt 1 antibody (4G9) serves as an invaluable tool for studying these complex interactions in various biological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Arnt 1 Antibody (4G9) References:

1. Molecular characterization and tissue distribution of aryl hydrocarbon receptor nuclear translocator isoforms, ARNT1 and ARNT2, and identification of novel splice variants in common cormorant (Phalacrocorax carbo).  |  Lee, JS., et al. 2007. Comp Biochem Physiol C Toxicol Pharmacol. 145: 379-93. PMID: 17337252
2. Carbohydrate-responsive element-binding protein (ChREBP) is a negative regulator of ARNT/HIF-1beta gene expression in pancreatic islet beta-cells.  |  Noordeen, NA., et al. 2010. Diabetes. 59: 153-60. PMID: 19833882
3. Evolutionary conserved regulation of HIF-1β by NF-κB.  |  van Uden, P., et al. 2011. PLoS Genet. 7: e1001285. PMID: 21298084
4. Hypoxia-inducible factor-1β (HIF-1β) is upregulated in a HIF-1α-dependent manner in 518A2 human melanoma cells under hypoxic conditions.  |  Mandl, M., et al. 2013. Biochem Biophys Res Commun. 434: 166-72. PMID: 23541582
5. Clock-controlled output gene Dbp is a regulator of Arnt/Hif-1β gene expression in pancreatic islet β-cells.  |  Nakabayashi, H., et al. 2013. Biochem Biophys Res Commun. 434: 370-5. PMID: 23567972
6. Expression of aryl hydrocarbon receptor 1 (AHR1), AHR1 nuclear translocator 1 (ARNT1) and CYP1 family monooxygenase mRNAs and their activity in chicken ovarian follicles following in vitro exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).  |  Antos, PA., et al. 2015. Toxicol Lett. 237: 100-11. PMID: 26043675
7. The action of HIF-3α variants on HIF-2α-HIF-1β heterodimer formation is directly probed in live cells.  |  Kim, SH., et al. 2015. Exp Cell Res. 336: 329-37. PMID: 26160453
8. Hypoxic Gene Signature of Primary and Metastatic Melanoma Cell Lines: Focusing on HIF-1β and NDRG-1.  |  Ercin, ME., et al. 2019. Balkan Med J. 37: 15-23. PMID: 31594284
9. Aryl hydrocarbon receptor nuclear translocators (ARNT1, ARNT2, and ARNT3) of white sturgeon (Acipenser transmontanus): Sequences, tissue-specific expressions, and response to β-naphthoflavone.  |  Doering, JA., et al. 2020. Comp Biochem Physiol C Toxicol Pharmacol. 231: 108726. PMID: 32081761
10. Effects of Myeloid Hif-1β Deletion on the Intestinal Microbiota in Mice under Environmental Hypoxia.  |  Han, N., et al. 2020. Infect Immun. 89: PMID: 33106294

The preferred Arnt 1 antibody is Arnt 1 Antibody (A-3): sc-17811.