Arnt 1 Antibody (0.N.330): sc-70383

Arnt 1 Antibody (0.N.330) is a mouse monoclonal IgG1 antibody that detects Arnt 1 in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Arnt 1, also known as aryl hydrocarbon receptor nuclear translocator, plays a crucial role in cellular signaling as an essential factor for aryl hydrocarbon receptor (AhR) nuclear translocation upon ligand binding. This process regulates genes involved in xenobiotic metabolism, including those encoding cytochrome P450 enzymes, which detoxify harmful compounds. Arnt 1 consists of 789 amino acids and features a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain, and two PAS domains, which are critical for dimerization and DNA binding. The structural integrity of Arnt 1 enables interaction with AhR and subsequent activation of target genes, highlighting Arnt 1′s importance in maintaining cellular homeostasis and responding to environmental toxins. Anti-Arnt 1 antibody (0.N.330) serves as an invaluable tool for researchers studying transcriptional regulation mechanisms and environmental pollutant effects on gene expression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Arnt 1 Antibody (0.N.330) References:

1. Zinc induces the accumulation of hypoxia-inducible factor (HIF)-1alpha, but inhibits the nuclear translocation of HIF-1beta, causing HIF-1 inactivation.  |  Chun, YS., et al. 2000. Biochem Biophys Res Commun. 268: 652-6. PMID: 10679259
2. Metabolic changes detected by in vivo magnetic resonance studies of HEPA-1 wild-type tumors and tumors deficient in hypoxia-inducible factor-1beta (HIF-1beta): evidence of an anabolic role for the HIF-1 pathway.  |  Griffiths, JR., et al. 2002. Cancer Res. 62: 688-95. PMID: 11830521
3. Glucose and endoplasmic reticulum calcium channels regulate HIF-1beta via presenilin in pancreatic beta-cells.  |  Dror, V., et al. 2008. J Biol Chem. 283: 9909-16. PMID: 18174159
4. Regulation of glucose metabolism-related genes and VEGF by HIF-1alpha and HIF-1beta, but not HIF-2alpha, in gastric cancer.  |  Song, IS., et al. 2009. Exp Mol Med. 41: 51-8. PMID: 19287200
5. HIF-1beta determines ABCA1 expression under hypoxia in human macrophages.  |  Ugocsai, P., et al. 2010. Int J Biochem Cell Biol. 42: 241-52. PMID: 19828131
6. MicroRNA-101 negatively regulates Ezh2 and its expression is modulated by androgen receptor and HIF-1alpha/HIF-1beta.  |  Cao, P., et al. 2010. Mol Cancer. 9: 108. PMID: 20478051
7. IFN-γ attenuates hypoxia-inducible factor (HIF) activity in intestinal epithelial cells through transcriptional repression of HIF-1β.  |  Glover, LE., et al. 2011. J Immunol. 186: 1790-8. PMID: 21199896
8. MicroRNA-103/107 is involved in hypoxia-induced proliferation of pulmonary arterial smooth muscle cells by targeting HIF-1β.  |  Deng, B., et al. 2016. Life Sci. 147: 117-24. PMID: 26827991
9. MiR-107 suppresses cell proliferation and tube formation of Ewing sarcoma cells partly by targeting HIF-1β.  |  Chen, J., et al. 2018. Hum Cell. 31: 42-49. PMID: 29075999
10. Next Generation SICLOPPS Screening for the Identification of Inhibitors of the HIF-1α/HIF-1β Protein-Protein Interaction.  |  McDermott, A., et al. 2024. ACS Chem Biol. 19: 2232-2239. PMID: 39312747

The preferred Arnt 1 antibody is Arnt 1 Antibody (A-3): sc-17811.