ARL15 CRISPR/Cas9 KO Plasmid (h): sc-412313
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARL15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARL15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARL15 CRISPR/Cas9 KO Plasmid (h) | sc-412313 | 20 µg | $397.00 |
Overview
ARL15 (ADP-ribosylation factor-like GTPase 15) is a small ARF-family GTPase implicated in regulating intracellular membrane trafficking and organelle-associated signaling, processes that influence receptor recycling, endosomal dynamics, and compartment-specific lipid handling. Through these roles, ARL15 is positioned to affect cellular metabolism and signal transduction pathways that depend on vesicular transport and plasma membrane composition. Human genetic studies have associated ARL15 loci with cardiometabolic traits, including adiposity- and insulin-related phenotypes, supporting investigation of ARL15 in metabolic homeostasis and related disease biology. Its expression and activity can therefore be leveraged to probe how trafficking-linked GTPase signaling interfaces with endocrine responses and nutrient-dependent cellular programs.
ARL15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ARL15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ARL15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ARL15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARL15 protein expression.
This CRISPR knockout system enables efficient generation of ARL15-deficient cell models for investigation of ARL15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ARL15 exon(s) critical for ARL15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ARL15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARL15 CRISPR/Cas9 KO Plasmid (h) and ARL15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ARL15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARL15 HDR Plasmid (h) and ARL15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ARL15 homology arms to support homology-directed repair at defined ARL15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.