ARID2 Double Nickase Plasmid (h): sc-401863-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARID2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARID2 Double Nickase Plasmid (h) and ARID2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARID2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARID2 Antibody (E-3): sc-166117
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARID2 Double Nickase Plasmid (h) | sc-401863-NIC | 20 µg | $410.00 | |||
ARID2 Double Nickase Plasmid (h2) | sc-401863-NIC-2 | 20 µg | $410.00 |
ARID2 (AT-rich interaction domain 2) encodes a DNA-binding subunit of the PBAF (SWI/SNF) ATP-dependent chromatin remodeling complex that helps position nucleosomes to regulate transcription, enhancer activity, and chromatin accessibility. Through these functions, ARID2 contributes to control of cell-cycle programs, lineage specification, and DNA damage responses by shaping gene expression networks across the genome. Loss or alteration of ARID2 perturbs epigenetic regulation and has been associated with aberrant transcriptional states observed in multiple tumor types, making it a useful target for studying chromatin remodeling dependencies. ARID2 is also leveraged as a model factor to dissect PBAF-specific mechanisms distinct from canonical BAF complexes in genome organization and transcriptional control.
ARID2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARID2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARID2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARID2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARID2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.