ARHGAP4 Double Nickase Plasmid (h): sc-407621-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARHGAP4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARHGAP4 Double Nickase Plasmid (h) and ARHGAP4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARHGAP4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARHGAP4 Antibody (G-6): sc-376251
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARHGAP4 Double Nickase Plasmid (h) | sc-407621-NIC | 20 µg | $410.00 | |||
ARHGAP4 Double Nickase Plasmid (h2) | sc-407621-NIC-2 | 20 µg | $410.00 |
ARHGAP4 encodes a Rho GTPase-activating protein that accelerates GTP hydrolysis on Rho-family GTPases, shaping actin cytoskeleton dynamics, membrane protrusion, and cell polarity. Through modulation of RhoA/Rac1/Cdc42 signaling, ARHGAP4 influences adhesion-dependent signaling, neurite outgrowth, and directional migration in hematopoietic and neural contexts. Dysregulated Rho GTPase signaling is broadly implicated in altered cell motility, immune cell function, and invasive phenotypes, making ARHGAP4 a useful node for mechanistic studies of cytoskeletal remodeling. ARHGAP4 perturbation is therefore relevant for investigating pathways that couple actin organization to signaling outputs and transcriptional programs in disease-associated cellular states.
ARHGAP4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARHGAP4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARHGAP4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARHGAP4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARHGAP4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.