ARHGAP28 Antibody (G-3): sc-365361
- ARHGAP28 Antibody (G-3) is a mouse monoclonal IgG1 κ ARHGAP28 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 583-599 within an internal region of ARHGAP28 of human origin
- ARHGAP28 Antibody (G-3) is recommended for detection of ARHGAP28 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
- Anti-ARHGAP28 Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ARHGAP28 Antibody (G-3) for WB applications. These reagents are now offered in bundles with ARHGAP28 Antibody (G-3) (see ordering information below).
ARHGAP28 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARHGAP28 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARHGAP28 Antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARHGAP28, also known as rho GTPase activating protein 28 or KIAA1314, is a 729 amino acid protein characterized by a single helical Rho-GAP domain. ARHGAP28 functions as a negative regulator of Rho-type GTPases, facilitating the conversion of active GTP-bound GTPases to their inactive GDP-bound state, which is essential for maintaining cellular signaling balance and preventing excessive cellular proliferation. ARHGAP28 is expressed at moderate levels in the ovary and kidney, with lower expression in skeletal muscle and spleen, and exists in four alternatively spliced isoforms. ARHGAP28 undergoes DNA damage-induced phosphorylation, potentially mediated by ataxia-telangiectasia mutated (ATM) or ATM and Rad3-related (ATR) kinases, which may influence regulatory functions in response to cellular stress. ARHGAP28 gene is located on human chromosome 18, a region associated with various genetic disorders, including Trisomy 18, Niemann-Pick disease, and hereditary hemorrhagic telangiectasia, highlighting ARHGAP28′s importance in both normal physiology and disease contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARHGAP28 Antibody (G-3) References:
- Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 2000. DNA Res. 7: 65-73. PMID: 10718198
- Characterisation of a 19-year-old 'long-term survivor' with Edwards syndrome. | Petek, E., et al. 2003. Genet Couns. 14: 239-44. PMID: 12872820
- A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complex. | Raghavan, SC., et al. 2004. Nature. 428: 88-93. PMID: 14999286
- Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. | Katoh, M. and Katoh, M. 2004. Int J Mol Med. 14: 333-8. PMID: 15254788
- Chromosome 18 aberrations and epilepsy: a review. | Grosso, S., et al. 2005. Am J Med Genet A. 134A: 88-94. PMID: 15690352
- Heterogeneity of mutations in the ferrochelatase gene in Italian patients with erythropoietic protoporphyria. | Aurizi, C., et al. 2007. Mol Genet Metab. 90: 402-7. PMID: 17196862
- Hereditary hemorrhagic telangiectasia. | Kamal, AH. and Prakash, UB. 2007. Mayo Clin Proc. 82: 1364. PMID: 17976356
- Linkage of Niemann-Pick disease type C to human chromosome 18. | Carstea, ED., et al. 1993. Proc Natl Acad Sci U S A. 90: 2002-4. PMID: 8446622
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARHGAP28 Antibody (G-3) | sc-365361 | 200 µg/ml | $316.00 | |||
ARHGAP28 Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-527361 | 200 µg Ab; 10 µg BP | $354.00 | |||
ARHGAP28 Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-532734 | 200 µg Ab; 20 µg BP | $354.00 | |||
ARHGAP28 Antibody (G-3) AC | sc-365361 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ARHGAP28 Antibody (G-3) HRP | sc-365361 HRP | 200 µg/ml | $316.00 | |||
ARHGAP28 Antibody (G-3) FITC | sc-365361 FITC | 200 µg/ml | $330.00 | |||
ARHGAP28 Antibody (G-3) PE | sc-365361 PE | 200 µg/ml | $343.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 488 | sc-365361 AF488 | 200 µg/ml | $357.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 546 | sc-365361 AF546 | 200 µg/ml | $357.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 594 | sc-365361 AF594 | 200 µg/ml | $357.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 647 | sc-365361 AF647 | 200 µg/ml | $357.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 680 | sc-365361 AF680 | 200 µg/ml | $357.00 | |||
ARHGAP28 Antibody (G-3) Alexa Fluor® 790 | sc-365361 AF790 | 200 µg/ml | $357.00 | |||
ARHGAP28 (G-3) Neutralizing Peptide | sc-365361 P | 100 µg/0.5 ml | $68.00 |