ARHGAP21 Double Nickase Plasmid (h): sc-403424-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARHGAP21 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARHGAP21 Double Nickase Plasmid (h) and ARHGAP21 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARHGAP21. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARHGAP21 Antibody (E-10): sc-390145
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARHGAP21 Double Nickase Plasmid (h) | sc-403424-NIC | 20 µg | $410.00 | |||
ARHGAP21 Double Nickase Plasmid (h2) | sc-403424-NIC-2 | 20 µg | $410.00 |
ARHGAP21 encodes a Rho GTPase-activating protein that downregulates Rho-family signaling by accelerating GTP hydrolysis, thereby shaping actin cytoskeletal remodeling and cell polarity. Through modulation of RhoA and related GTPases, ARHGAP21 influences processes including vesicular trafficking, adhesion dynamics, and directed migration, integrating cues from receptor- and integrin-linked pathways. Its regulatory role connects to broader signaling networks that coordinate membrane trafficking with cytoskeletal contractility. Dysregulated Rho GTPase signaling is frequently associated with altered motility and invasive phenotypes in cancer and with defects in vascular and immune cell function, motivating mechanistic studies of ARHGAP21.
ARHGAP21 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARHGAP21 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARHGAP21. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARHGAP21 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARHGAP21-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.