ARHGAP19 Double Nickase Plasmid (h): sc-406142-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARHGAP19 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARHGAP19 Double Nickase Plasmid (h) and ARHGAP19 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARHGAP19. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARHGAP19 Antibody (B-11): sc-398428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARHGAP19 Double Nickase Plasmid (h) | sc-406142-NIC | 20 µg | $410.00 | |||
ARHGAP19 Double Nickase Plasmid (h2) | sc-406142-NIC-2 | 20 µg | $410.00 |
ARHGAP19 encodes a Rho GTPase-activating protein that downregulates RHO family signaling by accelerating GTP hydrolysis, thereby tuning actin cytoskeleton organization and downstream effects on cell shape, adhesion, and motility. Through modulation of Rho-dependent pathways, ARHGAP19 contributes to spatiotemporal control of membrane dynamics and cytoskeletal remodeling during processes such as migration and immune cell trafficking. Altered Rho GTPase regulation is broadly linked to dysregulated proliferation, invasion, and inflammatory signaling, making ARHGAP19 a useful node for studying cytoskeleton-coupled signal transduction. Expression and functional perturbation of ARHGAP19 have been examined in contexts relevant to hematopoietic and immune biology, where Rho signaling influences differentiation and effector responses.
ARHGAP19 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARHGAP19 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARHGAP19. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARHGAP19 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARHGAP19-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.