Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ARH3 Double Nickase Plasmid (m): sc-430308-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARH3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ARH3 Double Nickase Plasmid (m) and ARH3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Adprhl2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ARH3 Antibody (A-7): sc-374162
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARH3 Double Nickase Plasmid (m) | sc-430308-NIC | 20 µg | $410.00 |
Adprhl2 encodes ARH3, a mono-ADP-ribosylhydrolase that reverses serine-linked ADP-ribosylation and related poly(ADP-ribose) metabolites generated during DNA damage signaling. By removing ADP-ribose from modified proteins, ARH3 helps tune PARP-dependent responses, maintains NAD⁺/ADP-ribose homeostasis, and supports genome stability under replication and oxidative stress. In mouse cells, ARH3 function intersects with chromatin regulation and stress-response pathways that influence cell survival and inflammation-related signaling. Dysregulation of ADP-ribosylation turnover is linked to neurodegenerative phenotypes and heightened sensitivity to genotoxic stress, making Adprhl2 a useful node for mechanistic studies of DNA repair and cellular resilience.
ARH3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Adprhl2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Adprhl2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Adprhl2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Adprhl2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.