ARH1 CRISPR/Cas9 KO Plasmid (m): sc-419017
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ARH1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ARH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARH1 CRISPR/Cas9 KO Plasmid (m) | sc-419017 | 20 µg | $397.00 |
Overview
Adprh encodes ARH1 (ADP-ribosylarginine hydrolase 1), a cytosolic enzyme that hydrolyzes arginine-linked mono-ADP-ribose from proteins and helps maintain ADP-ribosylation homeostasis. By reversing mono-ADP-ribosylation, ARH1 influences stress and DNA damage signaling, innate immune pathways, and protein function regulation through post-translational modification turnover. Disruption of ADP-ribose metabolism has been connected to altered inflammatory responses, genome maintenance defects, and dysregulated cell survival programs. In mouse models, Adprh provides a tractable entry point for investigating how reversible arginine ADP-ribosylation shapes cellular resilience and disease-relevant phenotypes.
ARH1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adprh gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adprh together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adprh open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ARH1 protein expression.
This CRISPR knockout system enables efficient generation of Adprh-deficient cell models for investigation of ARH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adprh exon(s) critical for ARH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adprh genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ARH1 CRISPR/Cas9 KO Plasmid (m) and ARH1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adprh locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ARH1 HDR Plasmid (m) and ARH1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adprh homology arms to support homology-directed repair at defined Adprh target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.