Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ArgRS CRISPR/Cas9 KO Plasmid (h): sc-409431
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ArgRS CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ArgRS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ArgRS Antibody (ZB-12): sc-100990
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ArgRS CRISPR/Cas9 KO Plasmid (h) | sc-409431 | 20 µg | $397.00 |
Overview
RARS encodes human arginyl‑tRNA synthetase (ArgRS), a cytosolic aminoacyl‑tRNA synthetase that ligates arginine to its cognate tRNA to ensure translational fidelity and efficient protein synthesis. ArgRS participates in core RNA metabolism and translation pathways and can functionally interface with multi‑synthetase complexes that coordinate protein biosynthesis with cellular stress and signaling programs. Disruption of aminoacyl‑tRNA synthetase activity has been linked to proteostasis imbalance and altered stress responses, making RARS a relevant gene for investigating mechanisms connecting translation control to cell viability and differentiation. RARS is also studied in the context of disease-associated variants affecting tRNA charging and downstream effects on proteome quality.
ArgRS CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RARS gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RARS together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RARS open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ArgRS protein expression.
This CRISPR knockout system enables efficient generation of RARS-deficient cell models for investigation of ArgRS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RARS exon(s) critical for ArgRS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RARS genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ArgRS CRISPR/Cas9 KO Plasmid (h) and ArgRS CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RARS locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ArgRS HDR Plasmid (h) and ArgRS HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RARS homology arms to support homology-directed repair at defined RARS target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.