Arginase 2/ARG2 Double Nickase Plasmid (h): sc-400639-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Arginase 2/ARG2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Arginase 2/ARG2 Double Nickase Plasmid (h) and Arginase 2/ARG2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARG2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Arginase 2/ARG2 Antibody (A-10): sc-393496
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arginase 2/ARG2 Double Nickase Plasmid (h) | sc-400639-NIC | 20 µg | $410.00 | |||
Arg2 Double Nickase Plasmid (h2) | sc-400639-NIC-2 | 20 µg | $410.00 |
Human ARG2 encodes arginase 2, a mitochondrial manganese-dependent enzyme that hydrolyzes L-arginine to L-ornithine and urea, linking amino acid catabolism with the urea cycle and polyamine/proline biosynthetic pathways. By competing with nitric oxide synthases for L-arginine, ARG2 can modulate nitric oxide availability and downstream redox, inflammatory, and vascular signaling programs. ARG2 activity integrates with mitochondrial metabolism and immune cell functional polarization, influencing cellular proliferation and stress responses through ornithine-derived polyamines. Dysregulation of arginine metabolism and altered ARG2 expression have been associated with cardiometabolic and inflammatory states and have been investigated in contexts such as tumor microenvironment nutrient competition and endothelial dysfunction.
Arginase 2/ARG2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.