ARFGAP1/3 Antibody (D-8): sc-365418
- ARFGAP1/3 Antibody (D-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 145-169 near the N-terminus of ARFGAP1/3 of mouse origin
- Anti-ARFGAP1/3 Antibody (D-8) is recommended for detection of ARFGAP1/3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ARFGAP1/3 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for ARFGAP1/3 Antibody (D-8) for WB applications. This reagent is now offered in a bundle with ARFGAP1/3 Antibody (D-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
ARFGAP1/3 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ARFGAP1/3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ARFGAP1/3 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ARFGAP1/3 plays a crucial role in vesicular trafficking by acting as a GTPase-activating protein for members of the ADP ribosylation factor (ARF) family, which are essential for protein and lipid transport within cells. ARFGAP1/3 facilitates GTP hydrolysis bound to ARF-1, promoting coat protein disassembly from Golgi-derived membranes and vesicles, a process vital for proper protein secretion and vesicle transport. ARFGAP1/3 is predominantly expressed in endocrine glands and testis, with significant expression also noted in adult brain, thymus, and lung, highlighting its importance in various physiological processes. ARFGAP1/3 activity is sensitive to phospholipids, which can influence its function in cellular signaling and trafficking pathways, making ARFGAP1/3 a key player in maintaining cellular homeostasis and communication.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ARFGAP1/3 Antibody (D-8) References:
- Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family. | Zhang, C., et al. 2000. Genomics. 63: 400-8. PMID: 10704287
- Functional characterization of novel human ARFGAP3. | Liu, X., et al. 2001. FEBS Lett. 490: 79-83. PMID: 11172815
- Paxillin-ARF GAP signaling and the cytoskeleton. | Turner, CE., et al. 2001. Curr Opin Cell Biol. 13: 593-9. PMID: 11544028
- Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1. | Yoon, HY., et al. 2004. Cell Signal. 16: 1033-44. PMID: 15212764
- A genome annotation-driven approach to cloning the human ORFeome. | Collins, JE., et al. 2004. Genome Biol. 5: R84. PMID: 15461802
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ARFGAP1/3 Antibody (D-8) | sc-365418 | 200 µg/ml | $322.00 | |||
ARFGAP1/3 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-522240 | 200 µg Ab, 40 µg BP | $361.00 | |||
ARFGAP1/3 Antibody (D-8) AC | sc-365418 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ARFGAP1/3 Antibody (D-8) HRP | sc-365418 HRP | 200 µg/ml | $322.00 | |||
ARFGAP1/3 Antibody (D-8) FITC | sc-365418 FITC | 200 µg/ml | $336.00 | |||
ARFGAP1/3 Antibody (D-8) PE | sc-365418 PE | 200 µg/ml | $349.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 488 | sc-365418 AF488 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 546 | sc-365418 AF546 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 594 | sc-365418 AF594 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 647 | sc-365418 AF647 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 680 | sc-365418 AF680 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 Antibody (D-8) Alexa Fluor® 790 | sc-365418 AF790 | 200 µg/ml | $364.00 | |||
ARFGAP1/3 (D-8) Neutralizing Peptide | sc-365418 P | 100 µg/0.5 ml | $69.00 |